氯胺酮对小鼠胃肠道蠕动功能的影响及机制  

Effects of ketamine on the gastrointestinal motility of mice

作　　者：吴奎霖[1] 李瑶函[1] 孙锶琦 郭小玮[1] 邹逸帆 张鑫磊[1] 仝坤[1] 秦霞[2] 

机构地区：[1]徐州医学院麻醉学院2012级,江苏徐州221004 [2]徐州医学院麻醉生理学教研室

出　　处：《徐州医学院学报》2016年第2期82-84,共3页Acta Academiae Medicinae Xuzhou

基　　金：江苏省高校自然科学研究面上项目（12KJD320005）;徐州医学院“振兴计划”项目

摘　　要：目的观察氯胺酮对小鼠胃肠道蠕动功能的影响及对小肠M受体的作用。方法将40只小鼠按给药方式分为生理盐水灌胃组、氯胺酮灌胃组、生理盐水腹腔注射组、氯胺酮腹腔注射组。给药2min后，经口灌人亚甲蓝溶液0．2ml。30min后将小鼠处死，测量亚甲蓝在肠管内移动距离和肠管全长，并计算亚甲蓝移动率。又将另外40只小鼠分为生理盐水组、阿托品组、氯胺酮组、阿托品＋氯胺酮组，腹腔注射给药，测定亚甲蓝移动率。结果氯胺酮灌胃组较生理盐水灌胃组亚甲蓝移动率更低（P〈0．05）。氯胺酮腹腔注射组较生理盐水腹腔注射组和氯胺酮灌胃组亚甲蓝移动率更低（P〈0．05）。同时，与阿托品组相比，氯胺酮组和阿托品＋氯胺酮组较氯胺酮组的亚甲蓝移动率明显降低（P〈0．05）。阿托品＋氯胺酮组较氯胺酮组亚甲蓝移动率无明显变化。结论氯胺酮对小鼠胃肠道蠕动有抑制作用，且与M受体无关。Objective To investigate the effects of ketamine on the gastrointestinal mobility of mice and M - R in the small intestine. Methods A total of 40 mice were divided into four groups according to their routes of administra- tion： a normal saline lavage group, a ketamine lavage group, a normal saline group by intraperitoneal injection, and a ketamine group by intraperitoneal injection. After administration of 2 min, 0.2 ml methylene blue solution was followed by oral administration. 30 min later, mice were sacrificed and their abdominal cavity was opened to measure the move- ment of methylene blue in the bowel and the full length of the intestinal, and to calculate the movement rate of methylene blue. Meanwhile, another 40 mice were divided into a normal saline group, an atropine group, a ketamine group, and an atropine and ketamine group. They were intraperitoneal injected with corresponding agents to measure the movement rate of methylene blue. Results The ketamine lavage group showed remarkably lower movement rate than the normal saline groups by lavage and intraperitoneal injection （ P 〈 0.05 ）. The ketamine group by intraperitoneal injection showed re- markably lower movement rate than the normal saline group by intraperitoneal injection and the ketamine lavage group （P 〈 0.05 ）. Meanwhile, compared with the atropine group, the movement rate was markedly reduced in the ketamine group and the atropine and ketamine group （P 〈 0.05 ）. Compared with the ketamine group, no significant change was found in the atropine and ketamine group. Conclusion Ketamine has inhibitory effects on the gastrointestinal motility in mice, without association with M - R.

关 键 词：氯胺酮 胃肠道 蠕动功能 M受体 

分 类 号：R570.5[医药卫生—消化系统]