ZmBK2L3在番茄果实中的特异表达及其功能研究  

Fruit-specific gene expression of ZmBK2L3 in tomato and its function

作　　者：杨潇怡[1] 隋媛[1] 唐晓凤[1] 曹徐绿 岳俊阳 刘永胜[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2016年第3期410-414,共5页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(31171179)

摘　　要：文章通过构建玉米BK2L3基因果实特异表达的过量表达载体,对转基因番茄果实进行研究,实现以基因工程手段延长番茄果实货架期的目的。利用逆转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)技术从玉米cDNA中扩增出ZmBK2L3基因全长,导入启动子为果实特异表达的TFM7植物表达载体pBI121上,经农杆菌介导转入野生型番茄中,获得转基因阳性植株。分别以野生型番茄和转基因番茄为材料,分析BK2L3在果实中的表达情况,观察绿熟期果实果皮细胞壁结构,测定红熟期果实的硬度、货架期和果皮纤维素质量比。结果表明:转基因番茄果实中BK2L3的表达量明显高于野生型果实。转基因番茄果实的硬度、果皮纤维素质量比和果皮厚度均比野生型有所提高,果实货架期明显增长。因此,BK2L3基因的过量表达与细胞壁代谢和货架期有关,可改良果实的品质。Fruit-specific overexpression vector of maize BK2L3 was constructed to investigate the effect of gene engineering on fruit firmness and shelf life. The full-length eDNA sequence of maize Zm BK2L3 gene was amplified by reverse transcription polymerase chain reaction（RT-PCR） and pBI121 BK2L3 overexpression construction driven by a fruit-specific promoter TFM7 was made to generate Agrobacerium mediated tomato transformants. In view of the transgenic tomatoes and the wild type tomatoes, the I3K2L3 expression in the fruit was analyzed, the pericarp cell wall structure of fruit at green ripe stage was observed, and the firmness, cellulose content and shelf life of fruit at red ripe stage were determined. The result of molecular analysis demonstrated a significant increase of BK2L3 expression in the transgenic tomatoes. The positive transgenic tomatoes displayed enhanced pericarp thickness. The firmness, cellulose content and shelf life of the transgenic red ripe fruits were higher than those of the wild type species. These results demonstrated that fruit-specific overexpression of maize BK2L3 in tomato could improve fruit quality in terms of cell wall metabolism and shelf life.

关 键 词：BK2L3基因 货架期 过量表达 果实特异表达 转基因番茄 遗传转化 

分 类 号：S641.2[农业科学—蔬菜学] Q786[农业科学—园艺学]