多发性骨髓瘤患者荧光原位杂交检测遗传学异常的回顾性分析  

Retrospective analysis of cytogenetic abnormalities detected by fluorescence in situ hybridization in patients with multiple myeloma

作　　者：蔡可[1] 黄红铭[1] 马亚男[1] 姜胜华[1] 丁润生[1] 陆伟[1] 沈毅[2] 孙中伟[1] 吴艳[1] 

机构地区：[1]南通大学附属医院血液内科,226001 [2]南通大学公共卫生学院,226001

出　　处：《实用医学杂志》2016年第5期778-781,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助(国家青年科学基金)项目(编号:81201857)

摘　　要：目的:探究多发性骨髓瘤(MM)患者荧光原位杂交(FISH)结果与临床特征之间的关系。方法:回顾性统计57例初诊MM患者的临床特征及FISH检测的遗传学异常(其中17例患者经CD138磁珠分选),分析其间的关系。结果:D13S319缺失和RB1缺失均与更高水平的乳酸脱氢酶(LDH)(P=0.024;P=0.018)及骨髓瘤细胞比例相关(P=0.027;P=0.013)。1q21扩增与更高水平的LDH量相关(P=0.030),与轻链型骨髓瘤发生相关(P=0.023)。IgH重排与患者发生肾功能损伤相关(P=0.009)。D13S319缺失、1q21扩增、RB1缺失及Ig H重排之间均两两相关(P<0.01)。结论 :FISH检测的MM患者遗传学异常与多种临床预后指标相关,对于更完善地评估患者的病情及预后有较大作用。Objective To investigate the relationship of the cytogenetic abnormalities detected by FISH in patients with MM and their clinical features. Methods FISH on bone marrow（BM） cells was performed in57 enrolled MM patients. Relationships between cytogenetic abnormalities and clinical features were analyzed.Results By statistical analysis, both D13S319 deletion and RB1 deletion were associated with high level of serum LDH（P = 0.024; P = 0.018） and BM plasma cells index（P = 0.027; P = 0.013）. 1q21 amplification was significantly associated with high level of LDH（P = 0.030） and the occurence of light chain type myeloma（P = 0.023）. Ig H rearrangement was associated with renal function damage（P = 0.009）. There were correlations among D13S319 deletion, RB1 deletion, 1q21 amplification and Ig H rearrangement（P 〈0.01）. Conclusion The genetic abnormalities detected by FISH in patients with MM were correlated with various clinical poor prognostic indicators, which can evaluate the condition and prognosis of patients more efficiently.

关 键 词：多发性骨髓瘤 荧光原位杂交 细胞遗传学异常 

分 类 号：R733.3[医药卫生—肿瘤]