云南高原湖泊杞麓湖冬季可培养酵母菌多样性分析  

Diversity of cultivable yeast in Qilu Lake in winter

作　　者：董明华[1] 李治滢[1] 周斌[1] 周巧[2] 严亚萍[1] 晋方佑[1] 李云霄[1] 杨丽源[1] 李绍兰[1] 

机构地区：[1]云南大学云南省微生物研究所,云南昆明650091 [2]昆明理工大学生物工程技术研究中心,云南昆明650000

出　　处：《微生物学报》2016年第4期603-613,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(31160006);云南省科技条件平台建设计划项目(2009DA002);国家微生物资源平台专项-面向微生物教学实验的专题服务项目(NIMR-2014-8)~~

摘　　要：【目的】探究云南杞麓湖酵母菌群落结构及其与环境因子的相互关系。【方法】采用原位培养方法对杞麓湖14个水样进行酵母菌分离,应用26S r DNA D1/D2区域序列分析,并结合形态及生理生化指标将对分离获得的酵母菌进行鉴定,运用软件bio-dap和Canoco分析酵母菌类群的丰富度及其与环境因子间的相互关系。【结果】从杞麓湖中分离得到321株酵母菌,鉴定为14个属27个种和1个潜在的新类群。Rhodosporidium kratochvilovae和出芽短梗霉(Aureobasidium pullulans)是优势种,分别为总菌株数的29.6%和16.8%。水体总磷含量是影响产色素红冬孢酵母属(Rhodosporidium)分布的重要环境因子,而p H为隐球酵母属(Cryptococcus)分布的一个重要选择条件。【结论】杞麓湖酵母菌具有较为丰富的群落多样性。[Objective] To investigate yeasts diversity in Qilu Lake and analyze the influence of environmental factors on yeast diversity.[Methods] Yeasts were isolated by in-situ cultivation and analyzed for the D1/D2 domain of large subunit（26S） ribosomal DNA and morphological characterization.We analyzed yeast species richness and species distribution in the Qilu Lake.[Results] In total 321 isolated yeasts were identified to 14 genera and 27 species.Rhodosporidium kratochvilovae and Aureobasidium pullulans were the dominating species in the lake,representing29.6% and 16.8% of the total strains,respectively.Principal component analysis（PCA） showed that the total phosphorus was an important environmental factor affecting the distribution of Rhodosporidium and the p H affected Cryptococcus yeasts distribution.[Conclusion] There was a high diversity of yeasts community in the Qilu Lake.

关 键 词：杞麓湖 酵母菌 多样性 环境因子 

分 类 号：Q938.8[生物学—微生物学]