茅尾海无瓣海桑内生细菌多样性及其细胞毒活性  

Diversity and cytotoxic activity of endophytic bacteria isolated from Sonneratia apetala of Maowei Sea

作　　者：李菲[1,2] 高程海[2] 竺利波[2] 余炼[1,2] 覃媚[1,2] 颜栋美[1] 

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西科学院广西近海海洋环境科学重点实验室,广西南宁530007

出　　处：《微生物学报》2016年第4期689-697,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金(81260480);广西科学研究与技术开发计划(14123001-7)~~

摘　　要：【目的】对无瓣海桑各组织器官内生细菌的分布特征、物种多样性及细胞毒活性进行分析。【方法】采用稀释涂布法分析无瓣海桑内生细菌的菌落形态及分布。利用16S r RNA基因序列进行系统发育分析,探讨无瓣海桑内生细菌的物种多样性。利用MTT法测试内生细菌培养液的乙酸乙酯提取物细胞毒活性。【结果】从各组织器官中分离出内生细菌38株,隶属12科21属(其中5属未定科),内生细菌数量和群落结构组成存在明显的组织特异性。在分离的菌株中,有5株菌与已有细菌物种典型菌株的全长16S r RNA基因相似性低于97%,代表着潜在新属或新种。5株内生细菌(R74、R71、S92、S85、S84)具有较强的细胞毒活性。【结论】无瓣海桑中可培养内生细菌的物种多样性丰富,潜藏较丰富的新物种资源,且含有较为丰富的活性菌株。[Objective] The purpose of this study was to study the distribution,diversity and cytotoxic activity of endophytic bacteria of Sonneratia apetala collected from Maowei Sea,Qinzhou city.[Methods] The 16 S r RNA gene sequencing and MTT were used to explore the diversity and cytotoxic activity of endophytic bacteria isolated from different organs and tissues of Sonneratia apetala.[Results] Total of 38 isolates were obtained.The result of diversity analysis showed that these isolates could be phylogenetically classified into 21 genera and 12 families,based on their16 S r RNA gene sequencing.Of them 5 were potential new genera or new genus.Five strains（R74,R71,S92,S85 and S84） had cytotoxic activity against human liver carcinoma Hep G2 cell line.[Conclusion] Endophytic bacteria of Sonneratia apetala are genetically diverse and most of them have abundant new bioactivities.

关 键 词：无瓣海桑 内生细菌 16S RRNA 物种多样性 细胞毒活性 

分 类 号：Q93[生物学—微生物学]