A血型亚型与糖基转移酶活性关系的初步研究  

Preliminary study on relationship between A blood subtype and glycosyl transferase activity

作　　者：史丽莉[1] 刘衍春[1] 陈妍[1] 马玲[1] 郑凌[1] 

机构地区：[1]江苏省血液中心输血研究室,南京210042

出　　处：《临床血液学杂志（输血与检验）》2016年第1期89-92,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:通过研究A亚型等位基因及其编码糖基转移酶的活性,进而了解不同A亚型的抗原性。方法:血型表型的定型采用微孔板法初检,试管法确认;糖基转移酶活性的检测采用血清学试管法;A血型亚型等位基因的检测采用DNA分子测序的方法。结果:在与抗A1不凝集的8例A亚型、24例A亚型B的标本中,无α-1、3-N-乙酰半乳糖基转移酶活性的分别为7例和24例;在与抗A1弱凝集的6例A型、13例AB型标本中,出现酶活性的分别为1例和4例。95.45%(42/44)的A亚型无α-1、3-N-乙酰半乳糖基转移酶活性。结论:从本次初步研究看绝大部分的A亚型无α-1、3-N-乙酰半乳糖基转移酶活性。不同A等位基因其A亚型的抗原性有差别。Objective：To investigate the different antigenicity of A subtype by analysing the A subtype alleles and activity of its glycosyl transferase.Method：A total of 10 012 specimens were tested by microtiter plates for ABO,then abnormal specimens were scanned and measured by tube test.Exon 6and exon 7of ABO gene were analyzed by PCR and sequence analysis.Result：In no agglutination of anti-A1 of 8cases of subtype A and 24 cases of speimens of AsubtypeB,noα-1,3-N-acetyl galactose glycosyl transferase activity were 7cases and 24 cases respectively.In weak agglutination of A subtype and AsubtypeB specimens,the enzyme activity cases were 1/6and4/13.95.45%（42/44）speimens of A subtype were lack of glycosyl transferase activity.Conclusion：The most part of A subtype ofα-1,3-N-acetyl galactose glycosyl transferase activity were no detected.And antigenicity of A subtype may have difference among different A alleles.

关 键 词：ABO血型 A亚型 等位基因 α-1、3-N-乙酰半乳糖基转移酶 

分 类 号：R457.1[医药卫生—治疗学]