血卟啉单甲醚荧光光谱对口腔黏膜癌的特异性诊断  

Hematoporphyrin monomethyl ether fluorescence spectrum specificity of diagnosis of the oral cancer

作　　者：赵严礼 吕茉扬[1] 毛立民[1] 

机构地区：[1]哈尔滨医科大学口腔医学院,150001

出　　处：《现代口腔医学杂志》2016年第2期102-104,共3页Journal of Modern Stomatology

摘　　要：目的在金黄地鼠口腔癌模型中确定血卟啉单甲醚荧光光谱对口腔黏膜癌的特异性诊断的可行性。方法选用成年金黄地鼠建立口腔黏膜癌模型,用9,10-二甲基1,2苯并蒽涂布于颊囊表面10周(n=20),小鼠处死前,组织中注射血卟啉单甲醚溶液,通过波长405nm的光纤光谱仪记录黏膜组织的药物荧光光谱,选用光谱参数S作为诊断标准,测量荧光光谱后,处死动物,对需测量的黏膜组织进行染色并记录组织病理学评价结果。结果 HMME染色的光谱显示正常组黏膜无明显染色,癌症组黏膜被染成不同程度的变化,两者呈现显著差异;癌性组织的S值(140≤S≤200)均高于正常组织(S≤10),且具有统计学意义。结论通过血卟啉单甲醚荧光光谱特异性荧光光谱可以作为诊断口腔黏膜恶性病变的一个标准。Objective This work is to determine the feasibility of HMME in diagnosing the oral mucosa tumors in the hamster cheek pouch model. Methods Adult hamsters were used for the study and a cheek pouch model was established. The 9,10-dimethyl-1,2-benzanthracene carcinogenesis was applied to one cheek pouch for 10 weeks（N=20）. Prior to sacrifice,HMME solution was injected into the tissues. The induced fluorescence spectra of the cheek tissues were recorded by a fiber spectrometer with excitation at 405 nm. a spectral parameter S was selected as diagnostic criterion. After fluorescence measurement,the animals were sacrificed and the measured tissues were collected.Histologicalstaining was performed and the results of histopathological evaluation were documented. Results Normal mucosa show no staining,the mucosa cancer group were stained with varying degrees of change.both of which showed a significant difference; The S value of cancerous tissue（140 ≤S ≤200） were higher than normal tissues（S ≤10）Conclusion The fluorescence spectroscopic method implemented by HMME can be used as a standard diagnostic oral mucosal malignant lesions.

关 键 词：血卟啉单甲醚(HMME) 光动力学诊断(PDD) 口腔黏膜 

分 类 号：R739.85[医药卫生—肿瘤]