低浓度二甲双胍对肝癌细胞线粒体形态结构及功能的影响  

Effects of low concentration of metformin on morphology and function of mitochondria of HepG2 cells

作　　者：陈纪涛[1] 陈良才[1] 贾小婷[2] 梁敏[1] 石波云[1] 刘季芳[1] 

机构地区：[1]广州医科大学附属第五医院,广州市510710 [2]广州医科大学附属肿瘤医院肿瘤研究所,广州市510095

出　　处：《实用医学杂志》2016年第6期875-878,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81402011);广东省科技计划项目(编号:2013B021800178);广东省教育厅青年创新人才项目(编号:2015001);广东省优秀青年教师项目(编号:B158030)

摘　　要：目的:研究低浓度二甲双胍对人肝癌Hep G2细胞线粒体形态结构及功能的影响。方法:选用人肝癌细胞株Hep G2作为研究对象,分别用0 mmol/L(对照组)、1 mmol/L(实验组)的二甲双胍对其进行处理,在低氧(1%)的恒温恒湿箱中孵育12 h后。采用Mitoview Orange染色及透射电镜,检测二甲双胍对Hep G2细胞线粒体形态结构及数目的影响;采用复合物Ⅰ活性检测试剂盒检测线粒体呼吸链复合物Ⅰ的活性。结果:Mitoview Orange染色发现,在低氧条件下,与对照组比较,低浓度二甲双胍对实验组Hep G2细胞线粒体形态结构及数目影响不大,两组间差异无统计学意义(P>0.05)。这与透射电镜观察结果相一致。但DAK法结果显示,实验组Hep G2细胞线粒体呼吸链复合物Ⅰ的活性明显低于对照组(P<0.05)。结论:低氧条件下,低浓度二甲双胍对肝癌Hep G2细胞线粒体形态结构及数目没有明显影响,但显著降低其线粒体活性。Objective To explore the potential impact of low concentration of metformin on the morphology and function of mitochondria of Hep G2 cells. Methods Hep G2 cells in experimental group and control group were treated with or without low concentration of metformin（1mM/L）, respectively. The cells were incubated for12 h in the incubator with constant temperature and humidity as well as 1% oxygen. Orange Mitoview was used to stain the mitochondria to detect the effects of the drug on its morphology and quantity. Transmission electron microscope was utilized to observe the effect of metformin on the ultrastructure of mitochondria. The mitochondrial respiratory chain complex I activity in Hep G2 cell was detected by Complex I Enzyme Activity Dipstick Assay Kit（DAK）. Results Orange Mitoview staining showed that low concentration of metformin had little effect on the morphology and number of mitochondria of cells in experimental group, and the difference between control and experimental group was not statistically significant（P〉0.05）. In addition, the result was further determined by transmission electron microscopy. However, DAK analysis showed that complex I activity of cells in experimental group was significantly lower than that in control group. Conclusion Under Hypoxia conditions, low concentration of metformin had no significant effect on the morphology and number of mitochondria of Hep G2 cells, but it significantly reduces the activity of mitochondria of Hep G2 cells.

关 键 词：肝肿瘤 二甲双胍 线粒体 

分 类 号：R735.7[医药卫生—肿瘤]