甘蓝型油菜湿害相关基因BnLDH-1的克隆和表达分析  

Cloning and Expressional Characterization of Wet Damage Related Gene BnLDH-1 from Brassica napus L.

作　　者：吴金海[1] 周玉堂 谢伶俐[1] 张学昆[3] 许本波[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]孝感市农业科学院,湖北孝感432000 [3]中国农业科学院油料作物研究所,湖北武汉432600

出　　处：《河南农业科学》2016年第3期53-57,66,共6页Journal of Henan Agricultural Sciences

基　　金：国家高技术研究发展计划(863计划)项目(2011AA10A104)

摘　　要：采用RACE技术从中双9号中克隆了甘蓝型油菜BnLDH-1基因全长cDNA序列和基因组序列。BnLDH-1基因的DNA序列为1 432 bp,包含1个内含子;cDNA序列全长1 319 bp,含有1个1 053 bp的ORF,其编码蛋白含350个氨基酸。经预测发现,BnLDH-1蛋白存在LDH-1保守结构域。qRT-PCR结果表明,湿害诱导BnLDH-1表达,但在不同耐湿性甘蓝型油菜品种中,BnLDH-1的诱导表达水平不同。相关分析表明,湿害胁迫后48 h的BnLDH-1表达量与12份测试的甘蓝型油菜材料的耐湿指数呈显著负相关,其相关系数为0.73。The BnLDH-1 gene cDNA and genomic sequences were cloned by the RACE from Zhongshuang No. 9. The genomic sequence of BnLDH-1 was 1 432 bp and contained one intron. The 1 319 bp BnLDH-1 cDNA had a 1 053 bpORF which encoded a polypeptide of 350 amino acids. The predicted results indicated that the BnLDH-1 protein contained conserved LDH-1 domain. qRT-PCR results indicated that BnLDH-1 transcription levels were induced by waterlogging,and the BnLDH-1 transcription level had significant difference among the Brassica napus L. with different waterlogging tolerance after waterlogging treatment. Correlation analysis indicated that BnLDH-1 transcription levels were significantly negatively correlated with waterlogging tolerance indices of 12 Brassica napus L. at 48 h after waterlogging treatment,and the correlation coefficient was 0. 73.

关 键 词：甘蓝型油菜 克隆 表达 乳酸脱氢酶 耐湿 

分 类 号：S565.4[农业科学—作物学] Q78[生物学—分子生物学]