小麦属物种ω-醇溶蛋白序列的克隆与分析  

Cloning and Analysis of Novel ω-gliadin Sequences from Triticum Species

作　　者：李增林[1] 吴丹丹[1] 李洪雨[1] 陈刚[1] 刘小娟[1] 甯顺腙[1] 刘登才[1] 张连全[1] 

机构地区：[1]四川农业大学小麦研究所,四川成都611130

出　　处：《麦类作物学报》2016年第3期268-272,共5页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(31271723;31201210);四川省杰出青年基金项目(2011JQ0016);四川省教育厅项目(14ZA0012)

摘　　要：为了挖掘ω-醇溶蛋白在小麦品质育种中的潜力,利用2对引物采用基因组PCR方法分别从阿拉拉特小麦（Triticum timopheevi Zhuk.var.araraticum）、栽培二粒小麦（Triticum turgidum ssp.dicoccon）、提莫菲维小麦（Triticum timopheevi）、栽培一粒小麦（Triticum monococcumssp.monococcum）和野生一粒小麦（Triticum monococcum var.boeoticum）5个小麦属物种中克隆出ω-醇溶蛋白序列,并对其进行序列分析。结果表明,本研究共克隆到6条新的ω-醇溶蛋白序列,归属于ARH、ATN和TRQ三种类型。这些新的ω-醇溶蛋白序列的长度为927~1 095bp。在这6个序列中,除KR082150拥有两个甲硫氨酸（Met）外,其余的均缺少含硫氨基酸。进化分析表明,本研究获得的6条ω-醇溶蛋白序列聚在了2个主要的分支,其中N端第一个氨基酸为丙氨酸（Ala）的ω-醇溶蛋白聚在了一个大的分支中,而TRQ类型的则聚在了另一个分支中。In order to exploit the potential value ofω-gliadin in wheat quality breeding,two pairs of primers were used to amplify theω-gliadins fromT.timopheevi Zhuk.var.araraticum,T.turgidum ssp.dicoccon,T.timopheevi,T.monococcum ssp.monococcum and T.monococcum var.boeoticum.Six novelω-gliadin sequences were isolated,which belonged to three types：ARH,ATN and TRQ.The lengths of 6sequences varied from 927 to 1 095 bp.Only KR082150 has two methionines,and the others have no cysteines and methionines.Phylogenetic analysis indicated thatω-gliadin sequences obtained in this study were mainly divided into 2branches.The ARH and ATN types ofω-gliadin sequences were clustered together in one branch,while TRQ type ofω-gliadin sequence was clustered in the other branch.

关 键 词：小麦属物种 ω-醇溶蛋白 基因克隆 系统进化 

分 类 号：S512.1[农业科学—作物学] S330