梓醇对成骨细胞-破骨细胞共育体系中破骨细胞活性、凋亡的影响及其机制研究  

Effect of Catalpol on the Activity and Apoptosis of Osteoclast in the Osteoblasts-osteoclasts Co-culture System and Its Mechanism Study

作　　者：赖满香[1] 陈侠[1] 冯娟[1] 何丽霞[2] 杨丽[2] 

机构地区：[1]广东食品药品职业学院,广州510520 [2]暨南大学药学院,广州510560

出　　处：《中国药房》2016年第10期1318-1321,共4页China Pharmacy

基　　金：广东省医学科学技术研究基金项目(No.B2015063);广东食品药品职业学院自然科学基金项目(No.2014YZ003)

摘　　要：目的：研究中药单体梓醇在成骨细胞（OB）-破骨细胞（OC）共育体系中对OC活性、凋亡的影响及其作用机制。方法：分别选用1~3 d龄和5~7 d龄SD乳鼠分离培养OB和OC,建立OB-OC共育体系。通过倒置显微镜观察0（空白对照）、0.05、0.5、5、50、100 mg/L梓醇培养48、72、96 h后OC的骨吸收陷窝数目,以反映OC活性;以0（空白对照）、0.05 mg/L梓醇培养细胞48、72、96h,检测OC中抗酒石酸酸性磷酸酶（TRACP）活性,计算OC的凋亡率;以0（空白对照）、0.05 mg/L梓醇培养细胞并检测OB中骨保护素（OPG）m RNA的表达。结果：在OB-OC共育体系中,0.05~50 mg/L梓醇作用下所形成骨吸收陷窝数目显著低于空白对照（P〈0.01）,表明梓醇可抑制OC活性,其中质量浓度为0.05 mg/L时作用最明显。与空白对照比较,0.05 mg/L梓醇作用后OC中TRACP的活性降低、OC的凋亡率升高（P〈0.05）;OB的OPG m RNA表达上调（P〈0.01）。结论：在OB-OC共育体系中,梓醇可抑制OC活性、诱导OC凋亡,其机制可能与上调OB的OPG m RNA表达有关。OBJECTIVE：To study the effect of catalpol on the activity and apoptosis of osteoclasts（OC） in the osteoblasts（OB）-OC co-culture system and its mechanism. METHODS：OB and OC were isolated respectively from the SD rats of 1-3 days and 5-7 days old to establish OB-OC co-culture system. After treated with 0（blank control）,0.05,0.5,5,50 and 100 mg/L catalpol for 48,72 and 96 h,the number of bone absorption lacuna for OC was observed by inverted microscope to reflect OC activity.After treated with 0（blank control）and 0.05 mg/L catalpol for 48,72 and 96 h,the activity of tartrate resistant acid phosphatase（TRACP）in OC was detected,and the apoptosis rate of OC was calculated. After treated with 0（blank control）and 0.05 mg/L catalpol,m RNA expression of osteoprotegerin（OPG）in OB was detected. RESULTS：In OB-OC co-culture system,the number of bone absorption lacuna in 0.05-50 mg/L catalpol groups was significantly lower than blank control group（P0.01）,indicating catalpol could inhibit OC activity,especially 0.05 mg/L catapol. Compared with blank control,0.05 mg/L catapol lowered the activity of TRACP but increased the apoptosis rate of OC（P0.05）;m RNA expression of OPG was up-regulated in OB（P0.01）. CONCLUSIONS：In OB-OC co-culture system,catalpol can inhibit the activity of OC and induce the apoptosis of OC,and its mechanism may be associated with the m RNA expression up-regulation of OPG in OB.

关 键 词：梓醇 成骨细胞 破骨细胞 共育体系 活性 凋亡 

分 类 号：R965[医药卫生—药理学]