姜黄素对A549细胞亚群SP和NON-SP的NF-κB、VEGF及Notch通路的影响  

Curcumin extract influence on tumor angiogenesis of A549 cell subsets SP and NON-SP cells

作　　者：李小江[1] 贾英杰[1] 张文治 张莹[1] 许文婷[3] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津神经外科研究所,天津300060 [3]天津中医药大学研究生院,天津300193

出　　处：《天津中医药》2016年第3期164-168,共5页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81001578);抗癌重大专项攻关计划(12ZCDZSY15800);天津市应用基础与前沿技术研究计划重点项目(14JCZDJC36900)

摘　　要：[目的]通过动物实验了解姜黄提取物-姜黄素抗肿瘤血管生成的具体作用机制。[方法]将40只BALB/C雄性裸小鼠分为4组,每组10只。分别为A组(SP亚群细胞姜黄素组)、B组(SP亚群细胞荷瘤对照组)、C组(NONSP亚群细胞姜黄素组)、D组(NON-SP亚群细胞荷瘤对照组)。A、B两组于实验前建立肺腺癌A549 SP细胞亚群荷瘤模型,C、D两组建立肺腺癌A549 NON-SP细胞亚群荷瘤模型,建立模型后观察16 d,于A组、C组小鼠腹腔注射姜黄素,隔天1次,B、D两组注射生理盐水。16 d后将小鼠称重后处死,剥离瘤块组织,比较各组瘤质量;免疫组化法检测肿瘤组织中血管生长因子(VEGF)、核因子-κB(NF-κB)的表达;逆转录-聚合酶链反应(RT-PCR)检测Notch1 m RNA含量。[结果]肺腺癌A549 SP细胞亚群荷瘤模型组小鼠瘤体与NON-SP细胞亚群荷瘤模型组小鼠相比体积较大;SP亚群细胞姜黄素组抑瘤作用及抗肿瘤血管生成优于NON-SP亚群细胞姜黄素组,两组相比差异具有统计学意义。[结论]姜黄素可以抑制肿瘤生长,考虑可能与其抑制NF-k B的表达,下调Notch1 m RNA含量,阻断Notch信号通路,抑制肿瘤组织中VEGF的表达有关。[Objective] Through the animal experiment further understand the main ingredient in turmeric extract curcumin-of tumor angiogenesis of the specific mechanism. [Methods] The 40 BALB / C mice were divided into four groups of male nude, one group is only10. group A（SP and group of cells curcumin group）, group B（SP and group of cells tumor-burdened control group）, group C（as SP and group of cells curcumin group）, group D（as SP and group of cells tumor-burdened control group）. A and B two groups in lung adenocarcinoma before the establishment A549 SP cells subsets tumor-burdened model, C, D two groups establish lung adenocarcinoma A549 cells subsets as SP tumor-burdened model, and after 16 days the model are observated, in group A, C intraperitoneal injection of mice curcumin, the next day 1, eight times, B and D two groups of injection saline. 16 days later executed mice, will tumor piece of tissue dissection, comparison of the heavy; immunohistochemical method to detect tumor tissue VEGF, CD34, NF-k B expression; PT-PCR detected Notch1 m RNA content. [Results] The group of cells SP curcumin group of suppression effect was better than the group of cells as SP curcumin group that cancer stem cells and tumor angiogenesis closely related. [Conclusion] Curcumin can inhibit tumor growth,and may inhibit the expression of NF-k B, down regulate the content of m RNA Notch, block the Notch1 signaling pathway and inhibit the expression of VEGF in tumor tissues.

关 键 词：姜黄素 血管生长因子 核因子-κB 人肺腺癌A549 NON-SP细胞 SP细胞 

分 类 号：R733[医药卫生—肿瘤]