长期酒精暴露对小鼠海马及大脑皮质神经细胞的影响  

Effects of chronic alcohol exposure on hippocampus and cerebral cortex neurons in mice

作　　者：崔占军[1] 赵凯冰[2] 邓锦波[1] 

机构地区：[1]河南大学医学院神经生物学研究所,河南开封475001 [2]开封大学医学部,河南开封475001

出　　处：《药学学报》2016年第4期573-579,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金面上项目(31070952)

摘　　要：探讨长期酒精暴露时,小鼠中枢神经系统神经细胞的数量、形态及其超微结构等的改变。选择60天左右的成年小鼠,建立长期酒精暴露动物模型。采用免疫细胞化学及DiI(1,1'-dioctadecyl-3,3,3',3'-tetramethy lindocarbocyanine perchlorate)散射等方法标记中枢神经系统神经细胞核抗原、树突棘及突触等结构。结果显示长期酒精暴露能够促进小鼠中枢神经系统中神经细胞凋亡,同时抑制神经干细胞的增殖,从而使中枢神经系统神经细胞数量减少。长期酒精暴露还可导致神经细胞的树突棘密度降低,神经细胞之间的连接结构——突触的数量减少,并且突触超微结构也可出现一定的改变。神经细胞及其树突棘的密度以及突触超微结构的改变提示神经细胞功能可能低下,它可能是酗酒引发神经系统损害的原因之一。This study was performed to investigate the changes of the number, morphology and ultrastructure of the central nervous system of mice during the long-term alcohol exposure. Mice at 60 days in age were used to establish the long-term alcohol exposure model. The structure of the central nervous system, such as nuclear antigen, dendritic spines and synapses, were labeled by the methods of immunocytochemistry and DiI（1,1＇- dioctadecyl-3,3,3＇,3＇-tetramethy lindocarbocyanine perchlorate） scattering. The results showed that prolonged alcohol exposure could promote apoptosis of nerve cells in the central nervous system, and inhibit the proliferation of neural stem cells, which reduced the number of nerve cells in the central nervous system. Long-term ethanol exposure can also lead to a decrease in the density of dendritic spines of neuron, a smaller number of synapses（connections between nerve cells）, and some changes in synaptic ultrastructure. The density of nerve cells and their dendritic spines, as well as the changes of synaptic ultrastructure, suggest that the function of nerve cells may be low.

关 键 词：酒精暴露 大脑皮质 激光共聚焦显微镜 免疫荧光 DiI散射 

分 类 号：R749.6[医药卫生—神经病学与精神病学]