加味香砂六君子汤对大鼠胃癌前病变胃黏膜Bax、Bcl-2、P53mRNA表达的影响  

Effect of Modified Xiangsha Liujunzi Decoction on Bax,Bcl-2,P53mRNA Expressions in Gastric Mucosa of Rats with Precancerous Lesions of Gastric Cancer

作　　者：李康[1] 李秋华[2] 

机构地区：[1]辽宁省肿瘤医院,辽宁沈阳110042 [2]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2016年第4期882-885,I0006,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省中医药临床学(专)科能力建设项目(2014-lnzyxzk-05)

摘　　要：目的:加味香砂六君子汤在胃癌前病变(PLGC)模型大鼠胃黏膜的Bax、Bcl-2、P53mRNA表达方面的影响及作用机理研究。方法:120只健康Wistar大鼠按照随机数字表法分为空白对照组和模型组、维霉素组、加味香砂六君子汤高、中及低剂量组,造模后给予药物干预治疗,通过免疫组化法,检测Bcl-2、Bax及P53mRNA表达水平。结果:加味香砂六君子汤各剂量组Bcl-2蛋白阳性表达率较模型组及维霉素组显著降低(P<0.05),各剂量组Bax蛋白阳性表达率较模型组及维霉素组明显增高(P<0.05)。各干预组p53mRNA蛋白表达不同程度降低,且明显低于模型组(P<0.05);其中加味香砂六君子汤中剂量组的作用效果最显著,与其他各组相比,差异显著(P<0.05)。结论:加味香砂六君子汤可调控胃癌前期病变大鼠胃黏膜Bcl-2、Bax表达水平,降低p53mRNA的表达,具有治疗PLGC的作用。Objective： To observe the impact of Modified Xiangsha Liujunzi Decoction on precancerous lesions（ PLGC） rat model＇s Bax,Bcl-2 and P53 mRNA expressions in gastric mucosa from the perspective of apoptosis and to explore the mechanism.Methods： 120 healthy Wistar rats were randomly divided into control group,model group,Vitacoenzyme group,Modified Xiangsha Liujunzi Decoction high,medium and low dose groups by random number table.After modeling,drug intervention was administered for six weeks,followed by immunohistochemical method for Bcl-2,Bax and P53 mRNA expression levels.Results： Modified Xiangsha Liujunzi Decoction high,medium and low dose groups＇ positive expressions of Bcl-2 protein in gastric mucosa compared with those of the model group and Vitacoenzyme group were significantly lower（ P〈0.05） while the positive rate of Bax protein expression compared with that of the model group and Vitacoenzyme group was significantly higher（ P〈0.05）.Each drug intervention group can reduce the expression of p53 mRNA in different degrees which was significantly lower than that of the model group（ P〈0.05）.Modified Xiangsha Liujunzi Decoction medium dose group was the most significant compared with the other groups（ P〈0.05）.Conclusion：Modified Xiangsha Liujunzi Decoction in the treatment of precancerous lesions can adjustable Bcl-2,Bax protein levels and reduce p53 mRNA to achieve clinical efficacy of treatment of PLGG.

关 键 词：加味香砂六君子汤 胃癌前病变 BAX BCL-2 P53MRNA 

分 类 号：R735.2[医药卫生—肿瘤]