尾吊小鼠感染空间诱变大肠杆菌炎症反应增强  

Increased inflammatory reaction in tail-suspension mice infected by E. coli in spaceglight

作　　者：姚静[1] 程江[2] 裴雪枫 王静宇[4] 王俊锋[5] 张学林[5] 刘长庭[5] 袁明[4] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]石河子大学第一附属医院检验科,新疆石河子832000 [3]辽宁医学院,辽宁锦州121001 [4]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094 [5]解放军总医院南楼呼吸科,北京100853

出　　处：《中国比较医学杂志》2016年第3期1-5,共5页Chinese Journal of Comparative Medicine

基　　金：国家重点基础研究发展计划(973计划)(2014CB744404)

摘　　要：目的观察空间诱变大肠杆菌感染尾吊模拟失重小鼠后炎症反应变化。方法将40只C57BL/6小鼠随机分为对照、对照染菌、尾吊及尾吊染菌组,采用ELISA和RT-q PCR方法分别检测小鼠血浆和肠道组织中炎症因子TNF-α、IL-1β、IL-6的含量及mRNA表达,HE染色观察小肠组织形态学的改变。结果血浆炎症因子ELISA及肠道组织炎症因子PCR结果显示,与对照组相比,实验组小鼠血浆及肠道组织中炎症因子TNF-α、IL-1β、IL-6的表达均升高,且尾吊染菌组最为显著(P<0.01或P<0.001);小肠组织HE染色结果显示,实验组小肠粘膜均出现不同程度的损伤,且尾吊染菌组最为严重。结论空间诱变大肠杆菌感染尾吊小鼠后可显著升高血浆及肠道组织中炎症因子表达,导致更严重的肠道黏膜屏障受损,提示尾吊模拟失重后感染空间诱变大肠杆菌可致机体的炎症反应增强。Objective To observe the changes of inflammatory reaction in tail-suspension mice after infected by E. coli in spaceflight. Methods 40 C57 BL /6 mice were randomly divided into four groups： control group（ Con）,control＋ E. coli T1-13 group（ Con ＋ T1-13）,tail suspension group（ TS）,tail suspension ＋ E. coli T1-13 group（ TS ＋ T1-13）. The inflammatory cytokines TNF-α,IL-1β and IL-6 production in plasma and mRNA level in intestinal tissue were detected by ELISA and RT-q PCR,and HE staining was used to represent the morphology changes in small intestine tissue. Results Compared with the control group, the expression of inflammatory cytokines in plasma and intestinal tissue of allexperimental groups were increased,and the TS ＋ T1-13 group was most significant（ P 〈 0. 01 or P 〈 0. 001）. HE staining showed that the small intestine mucosa in the experimental groups were damaged in different degrees,and the damage of TS＋ T1-13 group was most serious. Conclusions The E. coli from spaceflight increased significantly the expression of inflammatory cytokines in plasma and intestinal tissue from infected tail-suspension mice,and brought more serious damages to the small intestinal mucosal barrier,which suggested that the inflammatory reaction would be increased in tailsuspension mice infected by E. coli from spaceflight.

关 键 词：空间诱变大肠杆菌 模拟失重 炎症因子 肠道组织 

分 类 号：R-332[医药卫生]