大肠埃希菌致兔慢性输卵管炎症模型的建立  

The establishment of chronic salpingitis model of New Zealand rabbits caused by Escherichia coli

作　　者：冯子聪 张昭[2] 黎哲[2] 冯博仪 陈思艾 李君宇[1] 肖小敏[2] 

机构地区：[1]暨南大学国际学院临床医学系,广州510000 [2]暨南大学附属第一医院妇产科,广州510000

出　　处：《中国比较医学杂志》2016年第3期19-23,共5页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(81370676);暨南大学国家级大学生创新项目(201310559069);广东省科技计划项目(2012B32000007);广州市科技计划项目(7411785665675)

摘　　要：目的利用大肠埃希菌建立新西兰兔输卵管慢性炎症模型。方法制备3×109/m L的混合菌液(大肠埃希菌:金葡菌:链球菌按2∶1∶1体积混合)和3×108/m L大肠埃希菌混悬液。18只新西兰雌兔随机分三组,其中正常组2只、混合菌液实验组8只、大肠埃希菌液实验组8只。通过经阴道宫腔插管灌注术分别将混合菌液和大肠埃希菌液注入两组实验兔体内。于菌液宫腔灌注术后第5、10、15、20天观察大体标本及光镜下输卵管炎症形成情况。结果混合菌液组和大肠埃希菌液组兔均于灌注术第15天呈慢性输卵管炎症改变。结论两种建模方法均具有接近人体感染途径、成模率高、操作简便的优点,是建立输卵管慢性炎症动物模型的有效方法。Objective To investigate the possibility of utilizing the mixed bacteria liquid and the Escherichia coli（ E. coli） liquid to establish the chronic salpingitis model of New Zealand rabbits,respectively. Methods Taken as the study object,the un-pregnant New Zealand rabbits（ 4 ~ 5 years old） were randomly divided into three groups： the normal group,the mixed bacteria experimental group and the E. coli experimental group. The trans-vaginal intrauterine intubation operation was performed for the injection of the mixed bacteria liquid and the E. coli liquid. Visual observation was to evaluate the gross pathological changes of the salpingitis and the pelvic cavity. HE staining and the light microscope were used to observe the micro-pathological changes of salpingitis. Results On the 15 thday after modeling,increased pelvic effusion,dense peritoneal adhesion,interstitial hyperplasia and infiltration of lymphocytes were observed in both experimental groups.Conclusion Through the trans-vaginal intrauterine intubation operation,the chronic salpingitis model of New Zealand rabbits could be successfully established either by using the mixed bacteria liquid or by using the E. coli liquid.

关 键 词：慢性输卵管炎症模型 混合菌液 大肠埃希菌液 经阴道宫腔内插管灌注术 

分 类 号：Q-31[生物学] R-332[医药卫生]