肺腺癌A549绿色荧光裸鼠模型中肿瘤血管内皮细胞来源初探  

Research on the source of endothelial cells in tumor vessels by A 549 tumor model with GFP nude mouse

作　　者：方天[1] 胡若愚[2] 胡文娟[1] 刘彪[1] 尤金炜[1] 恽时锋[1] 

机构地区：[1]南京军区南京总医院比较医学科,南京210002 [2]东南大学附属中大医院胸心外科,南京210002

出　　处：《中国比较医学杂志》2016年第3期46-51,共6页Chinese Journal of Comparative Medicine

摘　　要：目的建立绿色荧光裸小鼠皮下接种肺腺癌A 549模型,研究肺腺癌肿瘤血管生成过程中血管内皮细胞的来源。方法建立肺腺癌A 549皮下接种GFP裸小鼠模型,应用免疫荧光染色法进行CD 31染色,标记肿瘤血管;采用荧光显微镜观察并拍摄肿瘤组织冰冻切片;免疫组化检测肿瘤组织血管中内皮细胞内GFP表达。结果免疫荧光实验结果显示:GFP蛋白和CD 31蛋白可共表达于部分间质血管;部分血管表达CD 31蛋白而不表达GFP蛋白。免疫组化实验结果:肿瘤组织中部分间质细胞的细胞质和内皮细胞的细胞质表达GFP蛋白。结论组成肿瘤新生血管的内皮细胞部分来源于裸鼠体内,部分来源于肿瘤细胞。Objective To explore the source of endothelial cells in tumor vessels by A 549 tumor model with GFP nude mouse. Methods To establish the A 549 lung cancer models with GFP nude mice,expression of CD 31 was determined by immunofluorescence to label tumor vessels; to observe and take a picture of the tumor frozen section by confocal microscopy and invert microscope; expression of GFP in tumor vessels was determined by immunohistochemistry.Result The results of immunofluorescence showed： Tumor interstitial vascular endothelial cells or endothelial cells clusters and micro-vascular lumen size and shape are clearly visible by immunofluorescence,and part of vessels with no obvious lumen or irregular lumen. We can see green fluorescent in tumor cells of tumor tissue and endothelial cells which form of tumor vessels. The results of immunohistochemistry showed： expression of GFP was determined in cytoplasm of tumor stromal cells and endothelial cells in tumor vessels. Conclusion The endothelial cells which formed tumor neovessels that derived from GFP nude mice partly and the other part derived from tumor cells.

关 键 词：肺腺癌A 549 血管生成 内皮细胞 肿瘤干细胞 

分 类 号：R-332[医药卫生]