微核试验的改进  

Experience exchange in vitro micronucleus assay

作　　者：马宝良[1] 鲁克庆 

机构地区：[1]兰州大学第二医院泌尿外科研究所甘肃省泌尿系统疾病研究重点实验室,兰州730030 [2]甘肃省泌尿系统疾病临床医学中心,兰州730030

出　　处：《中国比较医学杂志》2016年第3期86-88,共3页Chinese Journal of Comparative Medicine

摘　　要：目的将传统的骨髓采集方法与改良的方法进行比较,明确两种方法在细胞数量、组织污染情况和涂片染色背景的差异,为以后微核试验的应用提供参考。方法将处死小鼠的胸骨取出,一部分,使用传统的骨髓采集方法,将小鼠的骨髓挤压于含胎牛血清的载玻片上,常规涂片;另一部分,用1 m L注射器抽取胎牛血清100μL刺入胸骨中,将骨髓冲出,涂环形薄片。结果两种方法均能达到实验要求,但是改良骨髓采集法,采集的细胞数量更多,组织污染细胞少,制片背景更加清晰。结论与传统的骨髓采集方法相比较,改良的骨髓采集法更具优势,使微核试验的细胞计数简易。Objective To compare the conventional marrow collection with new marrow collection,in the number of cell,other tissues pollution and the background of the smear,providing the reference for future micronucleus test.Methods The mice was enthanasia and the sternums were taken. One group,using the conventional method of marrow collection,squeezing the marrow to the slide with fetal bovine serum; the other group,using 1-m L injector extracting fetal bovine serum 100μL,injecting into mice sternums and rushing out the bone marrow for circle smear. Results Two methods can meet the requirement of test,but the new marrow collection can acquire more number of cells,less the tissues pollution and more clear in the background of smear. Conclusions Comparing with the conventional marrow collection,the new method has more superiority to simplify the next cell counting.

关 键 词：骨髓采集 胎牛血清 细胞 小鼠 

分 类 号：R-332[医药卫生]