嗜热子囊菌光孢变种(Thermoascus aurantiacus var.levisporus)固态发酵姜苗产内切β-葡聚糖酶条件优化  

Conditions optimization of solid-state fermentation with ginger stem silage for production of endoglucanase by Thermoascus aurantiacus var. levisporus

作　　者：王冬梅[1] 张秀云[1] 薛梅[1] 郑文红[1] 

机构地区：[1]莱芜职业技术学院,山东莱芜271100

出　　处：《畜牧与兽医》2016年第3期37-42,共6页Animal Husbandry & Veterinary Medicine

基　　金：莱芜高新区科技创新与发展专项资金项目(2014)第36号

摘　　要：姜苗营养丰富,但作为饲料存在粗纤维含量高而消化吸收率低的难题。内切β-葡聚糖酶主要由真菌代谢产生,可有效降解纤维素中的葡聚糖。本研究在单因子条件的基础上,探讨应用Plackett-Burman和中心组合设计等方法得到嗜热子囊菌光孢变种(Thermoascus aurantiacus var.levisporus)固态发酵姜苗生产β-葡聚糖酶的最适发酵条件。结果表明,最佳培养基组成是姜苗70%(w/w)、豆粕30%(w/w)、初始含水量61.0%、尿素1.70%(w/w),KH2PO40.13%(w/w),Ca Cl20.1%(w/w)(固体培养基的总量)。在上述培养基中50℃培养72 h,β-葡聚糖酶活力可达682.8 U/g,与原始培养条件相比酶活提高了51.2%.Ginger stem silage is rich in nutrition,but difficult to digest as the feed because of the higher crude fiber. Endoglucanase is mainly from fungal metabolism and can degrade glucosan in fiber effectively. Based on the single factor conditions,the Plackett-Burman combined with the central composite design was applied to obtain the optimal fermentation conditions of endoglucanase from Thermoascus aurantiacus var. levisporus with ginger stem silage. The mixture of ginger stem silage 70%（ w / w）,soybean meal 30%（ w / w）,initial moisture 61%（ w/w）,urea 1. 70%（ w/w）,KH2PO40. 13%（ w / w） and Ca Cl20. 1%（ w / w） were proved to be the optimized medium. Fermentation was performed at 50 ℃ for 72 hours with the medium,the activity of endoglucanase reached 682. 8 U / g,which was increased about 51. 2% comparing with the original culture conditions.

关 键 词：嗜热子囊菌光孢变种 姜苗 固态发酵 内切β-葡聚糖酶 

分 类 号：Q81[生物学—生物工程]