不同低温驯化条件对玉米螟赤眼蜂低温贮藏的影响  

Effects of Different Cold Acclimation Conditions on Cold Storage in Trichogramma ostriniae

作　　者：张烨[1] 连梅力[1] 李唐[1] 朱文雅[1] 

机构地区：[1]山西省农业科学院植物保护研究所/农业有害生物综合治理山西省重点实验室,太原030031

出　　处：《中国生物防治学报》2016年第2期277-281,共5页Chinese Journal of Biological Control

基　　金：山西省农业科学院博士研究基金(YBSJJ1511);山西省农业科学院育种工程项目(llyzgc146);山西省回国留学人员科研资助(2012-108);山西省财政支农项目(NYST2015-05);山西省科技攻关计划(20080311002-6)

摘　　要：低温驯化是昆虫应对外界环境低温的重要生存策略,驯化温度、驯化时间和冷藏时间是低温驯化方法中的关键因子。本文采用正交试验方差分析法检验了不同驯化温度、驯化时间和冷藏时间对用米蛾卵培养的玉米螟赤眼蜂4℃冷藏后的影响,结果显示,驯化温度和驯化时间交互作用对玉米螟赤眼蜂冷藏后的出蜂情况影响显著,当15℃驯化10 d后冷藏,出蜂卵数达到峰值为66.75±3.50,其出蜂率为(64.91±2.04)%;当10℃驯化20 d后冷藏,出蜂卵数为56.75±4.84,出蜂率达到峰值为(75.50±6.78)%。冷藏时间对玉米螟赤眼蜂低温驯化后的出蜂情况影响显著,其出蜂卵数和出蜂率均在冷藏60 d和90 d达到最大,出蜂卵数分别为53.61±3.64、55.39±3.51,出蜂率分别为(66.07±4.17)%、(68.30±4.08)%。研究表明,驯化温度、驯化时间和冷藏时间对低温驯化效果均有一定影响,低温驯化能增强玉米螟赤眼蜂的耐寒性,适于其长期冷藏,本试验中的最佳低温驯化条件为15℃驯化10 d。该研究结果对玉米螟赤眼蜂的低温耐受性研究及低温贮藏技术探讨均具有重要的理论指导意义。Cold acclimation is an important survival strategy for insects to cope with cool environments. Acclimation temperature, acclimation duration and storage duration are the key factors in cold acclimation. Orthogonal test and variance analysis were used to determine the impacts of different acclimation temperature, acclimation duration and storage duration on storage of Trichogramma ostriniae at 4 ℃. The results showed that interaction between acclimation temperature and duration on emergence number and emergence rate was significant. The maximum emergence number was 66.75±3.50 and emergence rate was（64.91±2.04）% for cold acclimation for 10 days at 15 ℃. The maximum emergence rate was（75.50±6.78）% and emergence number was 56.75±4.84 for cold acclimation for 20 days at 10 ℃. Cold storage duration also significantly affected emergence. Emergence number and emergence rate reached the maximum when cold storage duration was 60 or 90 days. The emergence number was 53.61±3.64 and 55.39±3.51, and the emergence rate was（66.07±4.17）% and（68.30±4.08）%, respectively. The optimal conditions of cold acclimation were 10 days at 15 ℃. These results are of important theoretical significance for studies of low-temperature tolerance and cold storage in T. ostriniae.

关 键 词：低温驯化 玉米螟赤眼蜂 低温贮藏 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]