牙龈卟啉单胞菌脂多糖对EA.hy926细胞一氧化氮合酶基因表达及凋亡水平的影响  

The effects of P. gingivalis lipopolysaccharide on nitric oxide synthase gene expression and apoptosis of EA.hy926 cells

作　　者：王骏[1] 杨文蔚 贾岳 吴亚菲[1] 

机构地区：[1]四川大学华西口腔医学院牙周科,口腔疾病研究国家重点实验室,四川成都610041 [2]航空总医院口腔诊疗中心,北京100011

出　　处：《牙体牙髓牙周病学杂志》2016年第3期147-150,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金面上项目(81371150)

摘　　要：目的:初步研究牙龈卟啉单胞菌脂多糖(P.gingivalis,P.g-LPS)对人脐静脉细胞融合细胞株EA.hy926一氧化氮合酶(NOS)基因表达的影响,并探讨一氧化氮(NO)释放量与细胞凋亡间的关系。方法:体外培养EA.hy926细胞,选择不同浓度P.g-LPS(10、100、1 000μg/m L)诱导刺激,经过不同时间检测其诱导型一氧化氮合酶(i NOS)和内皮型一氧化氮合酶(e NOS)mRNA表达水平、释放的NO量及相应细胞凋亡水平。结果:P.g-LPS诱导EA.hy926细胞i NOS mRNA表达水平在1 h即可升高,4、12 h持续高表达,24 h表达水平回落,但仍高于空白组水平;e NOS mRNA表达水平则时持续下降。不同时间和浓度的P.g-LPS刺激诱导后NO产生量均升高。P.g-LPS诱导EA.hy926细胞,4 h开始出现早期凋亡增加,随后凋亡水平逐渐升高,4、12、24 h凋亡水平均高于空白组。结论:P.g-LPS浓度和时间依赖性地抑制EA.hy926细胞中e NOS mRNA的表达,促进i NOS mRNA的表达。P.g-LPS可能诱导EA.hy926细胞对NOS/NO的调控从而促进细胞凋亡。AIM： To study the influences of P. gingivalis lipopolysaccharide（ P. g-LPS） on oxide synthase（ NOS） gene expression,nitric oxide（ NO） release and apoptosis of EA. hy926 cells. METHODS： The EA. hy926 cells were cultured in vitro and treated with P. g-LPS at 10 μg / m L,100 μg / m L and 1 000 μg / m L respectively. The mRNA expression of iNOS and e NOS,NO release and apoptosis were detected after different time periods of treatment.RESULTS： After P. g-LPS induction of EA. hy926 cells,i NOS mRNA level increased 1 h after treatment,remained stable until 12 h,and declined after 24 h without backing to that of the control group. e NOS mRNA level was decreased by P. g- LPS treatment. P. g- LPS of different concentrations at different treatment periods induced NO release. Apoptosis of EA. hy926 cells appeared after 4 h P. g-LPS treatment,and the apoptosis rate in P. g-LPS groups was higher than that of the control group at 4 h,12 h and 24 h. CONCLUSION： P. g-LPS may upregulate of i NOS mRNA and downregulate e NOS mRNA of of EA. hy926 cells in a dose- dependent manner. P. g- LPS can promote EA. hy926 cell apoptosis through NOS / NO regulation.

关 键 词：牙龈卟啉单胞菌 脂多糖 一氧化氮 细胞凋亡 

分 类 号：R780.2[医药卫生—口腔医学]