微流控芯片的制备及其对糖类物质的检测  

Preparation of Microfludic Chip for Sugar Detection

作　　者：皮晓强[1,2] 王升高[1,2] 刘星星[1,2] 张维[1,2] 陈睿[1,2] 崔丽佳[1,2] 

机构地区：[1]武汉工程大学材料科学与工程学院,湖北武汉430074 [2]湖北省等离子体化学与新材料重点实验室(武汉工程大学),湖北武汉430074

出　　处：《武汉工程大学学报》2016年第1期52-55,共4页Journal of Wuhan Institute of Technology

基　　金：湖北省科技支撑计划(2015BAA093);国家自然科学基金(51272187);湖北省自然科学基金(2013CFA012)

摘　　要：利用软光刻技术,在聚二甲基硅氧烷(PDMS)上制备了微流通道,通过光刻-湿法刻蚀和磁控溅射镀膜技术制备出了集成有金属膜电极的玻璃基片,将二者经过等离子体处理,进行键合后获得了集成有电化学检测器的PDMS-玻璃微流控芯片.以市售蜂蜜为待测物质,研究了该芯片对蜂蜜中糖类物质的分离检测性能.结果表明:在分离电压为850 V,电解液为50 mmol/L氢氧化钠条件下,使用该PDMS玻璃微流控芯片成功地在3 min内实现了对葡萄糖、蔗糖和果糖的有效分离和检测.The micro channels on the surface of Polydimethylsiloxane（PDMS）were prepared by soft lithogra-phy technology,and the glass substrate integrated with metal membrane electrode was prepared by lithography-wet etching and magnetron sputtering coating technology. After the surface oxygen plasma treatment of PDMSand glass substrate,the PDMS- glass microfluidic chip was prepared through reversible bonding. Finally,thesugar detection performance of the microfluidic chip was analyzed using the honey from a market as a testingmaterial. The result shows that the glucose,sucrose and fructose in honey can be successfully separated anddetected in 3 min at the running voltage of 850 V and 50 mmol/L sodium hydroxide solution

关 键 词：微流控芯片 磁控溅射 电泳分离 电化学检测 糖 

分 类 号：R284.1[医药卫生—中药学]