4′,5,7-三羟基异黄酮对LPS诱导的人主动脉内皮细胞ephrinB2及IL-6 mRNA表达的影响  

Effects of 4′,5,7 trihydroxyisoflavone on mRNA expression of ephrinB2 and IL-6 induced by LPS in human aortic endothelial cells

作　　者：姜念[1] 周雪冰[1] 欧刚卫[1] 陆红玲[1] 

机构地区：[1]遵义医学院,贵州遵义563000

出　　处：《现代医药卫生》2016年第7期966-968,共3页Journal of Modern Medicine & Health

基　　金：贵州省科技厅联合基金重点项目(黔科合J字LKZ[2013]14)

摘　　要：目的探讨4′,5,7-三羟基异黄酮对脂多糖(LPS)诱导人主动脉内皮细胞(HAEC)标记物ephrinB2及炎症因子白介素-6(IL-6)mRNA表达的影响。方法体外培养HAEC,分别用浓度为0.1、1.0、10.0、100.0、1 000.0μg/m L的LPS刺激HAEC 4 h,建立HAEC损伤模型。将培养的人主动脉内皮细胞分为模型组(LPS 0.1μg/m L),对照组,4′,5,7-三羟基异黄酮低(1.0μm/m L)、中(10.0μm/m L)、高(100.0μm/m L)浓度组。MTT法检测细胞的活力,Real-time PCR法检测HAEC ephrinB2及IL-6 mRNA表达情况。结果除10.0、100.0μg/L LPS组外,其余各浓度组与对照组比较,均能使HAEC的活力降低,差异均有统计学意义(P<0.05);LPS能促进ephrinB2及IL-6 mRNA的表达;4′,5,7-三羟基异黄酮中、高浓度组可促进HAEC细胞活力增加,差异均有统计学意义(P<0.05);4′,5,7-三羟基异黄酮低、中浓度组可明显降低ephrinB2及IL-6 mRNA的表达,差异均有统计学意义(P<0.01)。结论适当浓度的4′,5,7-三羟基异黄酮对LPS诱导的HAEC损伤有一定的保护作用。Objective To study the effects of 4′,5,7 trihydroxyisoflavone on mRNA expression of ephrinB2 and IL-6 in duced by LPS in human aortic endothelial cells（HAEC）. Methods Human aortic endothelial cells were cultured in vitro and0.1,1.0,10.0,100.0,1000.0 μg/m L of LPS was to stimulate HAEC for 4 h. The HAEC injury model was established. Then the cultured HAEC were divided into the model group（0.1 μg/m L LPS）,low,middle and high 4′,5,7 trihydroxyisoflavone concentra tion groups（1.0,10.0,100.0 μm/m L respectively）;the cell vitality was detected by MTT assay. The mRNA expressions of ephrinB2 and IL-6 were detected by reverse transcription polymerase chain reaction（RT-PCR）. Results Except for the 10.0,100.0 μg/m L LPS groups,the rest other concentration groups all could reduce the vitality of HAEC compared with the control group,the differences were statistically significant（P〈0.05）,LPS could promote the mRNA expression of ephrinB2 and IL-6;the middle and high concentration 4′,5,7 trihydroxyisoflavone groups HAEC could promote the increase of HAEC vitality with statis tical difference（P〈0.05） and the low and middle concentration 4′,5,7 trihydroxyisoflavone groups could significantly reduce the mRNA expression of ephrinB2 and IL-6 with statistical difference（P〈0.01）. Conclusion Appropriate concentration of 4 ′,5,7 trihdroxyisoflavone has a certain protective effect to LPS induced HAEC injury.

关 键 词：异黄酮类 内皮细胞 白细胞介素6 脂多糖类 细胞增殖 炎症 EPHRINB2 

分 类 号：R730.2[医药卫生—肿瘤]