免疫杀伤细胞治疗对恶性肿瘤患者调节性T细胞的影响  

Effect of immune killer cells therapy on regulatory T cells in patients with malignant tumor

作　　者：韩兆东[1] 阮月芹[2] 韩晓通 梁葵香[4] 孙其静 李卫[1] 

机构地区：[1]滨州医学院附属医院中心实验室,山东滨州256603 [2]滨州医学院附属医院检验科,山东滨州256603 [3]滨州医学院附属医院疼痛科,山东滨州256603 [4]滨州医学院附属医院产科,山东滨州256603

出　　处：《现代医药卫生》2016年第7期972-974,977,共4页Journal of Modern Medicine & Health

基　　金：滨州医学院科技计划项目(BY2012KJ13)

摘　　要：目的探讨免疫杀伤细胞治疗对恶性肿瘤患者CD4+CD25+CD127LOW、CD8+CD28-调节性T细胞的影响。方法选择2011年5月至2014年4月该院肿瘤内科收治的接受免疫杀伤细胞治疗的恶性肿瘤患者50例作为研究对象(肿瘤组),同时选择该院健康体检者30例为对照组。采用流式细胞术检测所有受检对象治疗前后外周血CD4+CD25+CD127LOW和CD8+CD28-调节性T细胞的含量。结果肿瘤组治疗前外周血CD4+CD25+CD127LOW/CD4+、CD8+CD28-/CD8+(11.48%±1.44%、19.69%±5.01%)明显高于对照组(4.65%±0.75%、15.35%±4.71%),差异有统计学意义(P<0.05)。肿瘤组治疗后外周血CD4+CD25+CD127LOW/CD4+、CD8+CD28-/CD8+(5.55%±0.81%、16.15%±4.61%)明显低于治疗前,差异均有统计学意义(P<0.05﹚;略高于对照组,但差异无统计学意义(P>0.05)。结论免疫杀伤细胞治疗恶性肿瘤,能够降低肿瘤患者外周血CD4+CD25+CD127LOW、CD8+CD28-调节性T细胞水平,纠正机体免疫失衡状态,提高免疫功能。Objective To investigate the effect of immune killer cells therapy on CD4＋CD25＋CD127LOWand CD8＋CD28-regulatory T cells in the patients with malignant tumor. Methods Fifty patients with malignant tumor treated by the immune killer cells therapy in the oncology department of our hospital from May 2011 to April 2014 were taken as the research subjects（tumor group） and 30 individuals undergoing the healthy physical examination were selected as the control group. Flow cytometry was used to detect the peripheral blood CD4＋CD25＋CD127LOWand T regulatory CD4＋cells with CD8＋CD28-and CD8＋. Results The peripheral blood CD4＋CD25＋CD127LOW/CD4＋and CD8＋CD28-/CD8＋before treatment in the tumor group were 11.48%±1.44%and 19.69%±5.01%,which were significantly higher than 4.65%±0.75% and 15.35%±4.71% in the control group,the differences were statistically significant（P0.05）. The peripheral blood CD4＋CD25＋CD127LOW/CD4＋and CD8＋CD28-/CD8＋after treatment in the tumor group were 5.55%±0.81% and 16.15%±4.61%,which were significantly lower than before treatment with statistical differences;but slightly higher than those in the control group without statistical differences（P〉0.05）. Conclusion Immune killer cells in treating malignant tumor can reduce the peripheral blood CD4＋CD25＋CD127LOW,CD8＋CD28-regulatory T cells level,corrects the immune imbalance state and improves the immune function.

关 键 词：杀伤细胞 T淋巴细胞 调节性 肿瘤 抗原 CD4 抗原 CD8 抗原 CD28 流式细胞术 

分 类 号：R-05[医药卫生]