1,8-桉油素预处理对Aβ_(25～35)诱导大鼠皮层神经元炎症反应的影响  

Effect of 1,8-cineol pretreatment on inflammatory response induced by Aβ_(25～35) in primary cultured cortical neurons

作　　者：王琳[1] 赵春贞[1] 

机构地区：[1]潍坊医学院药理学教研室应用药理学实验室,山东潍坊261053

出　　处：《中国老年学杂志》2016年第7期1540-1541,共2页Chinese Journal of Gerontology

基　　金：国家自然科学基金专项基金项目(81341137);潍坊市科技局项目(201301071)

摘　　要：目的探讨1,8-桉油素预处理对Aβ（25 ～ 35）诱导原代培养大鼠皮层神经元炎症反应的影响。方法原代培养大鼠皮层神经元,随机分组。采用噻唑蓝（MTT）还原反应观察神经元损伤;以Aβ（25 ～ 35）处理24 h诱导神经元损伤;酶联免疫吸附法（ELISA）检测上清液中肿瘤坏死因子（TNF）-α、白介素（IL）-1β及半胱氨酰白三烯（Cys LTs）释放水平。结果 20μmol/L的Aβ（25 ～ 35）处理神经元细胞24 h可显著降低神经元活性,增加TNF-α、IL-1β及Cys LTs的释放,1,8-桉油素10μmol/L预处理后可明显抑制Aβ（25 ～ 35）引起的细胞毒性效应,并抑制上述炎症因子及Cys LTs的释放。结论 1,8-桉油素对Aβ（25 ～ 35）诱导的神经元损伤具有保护作用,与抑制TNF-α、IL-1β及Cys LTs的释放有关。Objective To investigate the effect of 1,8-cineol against Aβ（25 ～ 35）-induced inflammatory response in rat primary cultured neurons. Methods Rat primary cultured cortical neurons were randomly divided into control,Aβ（25 ～ 35）（ 20 μmol/L）,low dose of 1,8-cineol（ Aβ（25 ～ 35）20 μmol / L ＋ 1,8-cineol 1 μmol / L）,high dose of 1,8-cineol（ Aβ（25 ～ 35）20 μmol / L ＋1,8-cineol 10 μmol / L） groups. Cell viability was evaluated by 3-（ 4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide（ MTT） reduction assay. Neuron injury was induced24 h after Aβ（25 ～ 35） treatment. Releases of TNF-α,IL-1β and Cys LTs in the supernatant were determined by ELISA. Results 20 μmol / L of Aβ（25 ～ 35） incubated for 24 h significantly reduced neuronal activity,and increased TNF-α,IL-1β and Cys LTs releases. Pretreatment with 1,8-cineol obviously inhibited cytotoxicity and decreased the level of above cytokines and Cys LTs releases. Conclusions 1,8-cineol has a protective effect on Aβ（25 ～ 35） induced neuron injury,probably relates with inhibition of TNF-α,IL-1β and Cys LTs releases.

关 键 词：1 8-桉油素 阿尔茨海默病 炎症 细胞因子 

分 类 号：R749.1[医药卫生—神经病学与精神病学]