运输时间和MS-222浓度对翘嘴鲌皮质醇、乳酸及氧气袋内水质的影响  

Effect of transport time and MS-222 concentration on cortisol,lactate levels of juvenile topmouth culter and the water quality indexes in O_2-aerated bags

作　　者：赵忠波[1] 胡培培 刘汝鹏[1] 胡伟华[1] 樊启学[1] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]湖北五源农业发展有限公司,湖北荆州434311

出　　处：《淡水渔业》2016年第2期94-98,共5页Freshwater Fisheries

基　　金：国家科技支撑计划项目"长江中下游特色种类规模化繁育及健康养殖技术"(2012BAD25B06)

摘　　要：釆用MS-222浓度为0（C0组）、5 mg/L（C1组）、10 mg/L（C2组）、15 mg/L（C3组）、20 mg/L（C4组）,运输时间为0、2、4、6、8、10 h,运输密度为33 g/L,采用5×6双因素实验,氧气袋运输翘嘴鲌（Culter alburnus）稚鱼,研究了运输时间和MS-222浓度对翘嘴鲌全鱼皮质醇、乳酸含量及氧气袋内水质的影响。结果显示：翘嘴鲌全鱼皮质醇、乳酸水平随运输时间呈现先升高后降低的趋势,随麻醉剂浓度的增加而显著降低;C1-C4组的DO、p H随运输时间呈现先降低后升高的趋势,C0组则随运输时间的增加显著降低,各组的DO和p H随MS-222浓度的增加而显著增加;C1-C4组的氨氮（TAN）和游离CO_2浓度随运输时间呈现先升高后降低的趋势,而C0组则随运输时间的增加显著升高,各组的TAN和游离CO_2浓度随MS-222浓度的增加而显著降低。时间和浓度的交互作用都显著。运输时间T〈6 h时,MS-222刺激翘嘴鲌,不利于运输,运输时间T〉6 h时,MS-222能显著降低其应激水平及代谢强度,其中C4组皮质醇、乳酸含量最低,C3、C4组水质条件最好,其适宜的麻醉剂浓度为15-20 mg/L。This study which used 5 × 6 factorial design was to determine the effect of transport time（ 0,2,4,6,8,10h） and MS-222 concentration（ 0（ C0）,5 mg / L（ C1）,10 mg / L（ C2）,15 mg / L（ C3）,20 mg / L（ C4）） on whole body cortisol,lactate levels of juvenile topmouth culter（ Culter alburnus） and the water quality indexes in the O_2-aerated bags used for transporting the fish. At the end of the experiment,the whole body cortisol and lactate levels of juvenile topmouth culter increased during the first 2 hours,then it decreased with the increasing of the transport time,while the two levels significantly decreased with the increasing of MS-222 concentration. Dissolved oxygen（ DO） and p H values in group C1-C4 declined during the first 6 hours of transport,then increased with transport time,while DO and p H value in group C0 declined with the increasing of transport time. Besides,DO and p H values in groups C0-C4 significantly increased with the increasing of MS-222 concentration. Total ammonia-nitrogen（ TAN） and CO_2（ aq） levels in groups C1-C4 increased during the first 8 hours,then decreased with the increasing of transport time,and the two indexes significantly decreased with the increasing of the MS-222 concentration. While,TAN and CO_2（ aq） levels in group C0 increased with time oftransport. The significant interaction between transport time and MS-222 concentration on cortisol,lactate levels and the water quality indexes was observed. In conclusion,our results suggested that an obvious irritant action of MS-222 on juvenile topmouth culter was observed during the first 6 h of transport. When the transport time of juvenile topmouth culter was longer than 6 hours,the appearance of MS-222 would reduce the stress response and metabolism rate of the topmouth culter,and the optimal MS-222 concentration would be 15 - 20 mg / L according to the minimum of cortisol and lactate level in group C4.

关 键 词：运输时间 MS-222浓度 翘嘴鲌(Culter alburnus) 全鱼皮质醇水平 全鱼乳酸水平 水质 

分 类 号：S981.1[农业科学—捕捞与储运]