一株红椒软腐病病原菌的分离鉴定及致病性研究  

Isolation,Identification and Pathogenicity of a Soft Rot Pathogen from Sweet Peppers

作　　者：张伟[1] 魏蜜[1] 张少琴[1] 傅本重[1] 王立华[1] 鲁旭东[1] 

机构地区：[1]湖北工程学院生命科学技术学院特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000

出　　处：《福建农业学报》2016年第1期40-45,共6页Fujian Journal of Agricultural Sciences

基　　金：湖北省教育厅中青年人才项目(Q20152707);国家自然科学基金(31200488);湖北省重点实验室开放基金重点项目(2016K07)

摘　　要：从湖北孝感地区红椒软腐病样中筛选分离获得1株软腐病原菌HBEU-9,在实验室条件下能快速侵染健康红椒组织,并能引起蔬菜田种植的红椒软腐病症发生。进一步对比分析该株病原菌对青椒、莴苣、土豆、萝卜、瓠瓜、白菜的致病性,发现HBEU-9是一株对常见蔬菜具有广谱致病性及致病能力强的软腐病原菌。对该菌株进行生理生化和分子鉴定,依据病原菌的形态特征,并结合其16SrDNA序列分析,将病原菌鉴定为菊欧文氏菌(Erwinia chrysanthemi,Ech)。采用抗生素对该病原菌进行拮抗作用分析,结果表明,相较其他抗生素,庆大霉素即使在较低浓度60μg·mL^(-1)时抗菌效果可达最佳。A pathogen,HBEU-9,was isolated from the soft rotted samples of sweet peppers in Xiaogan,Hubei.In vitro,inoculated under the laboratory conditions,it could macerate tissues of sweet peppers.In the field,it infected the peppers with the soft rots.Pathogenicity of HBEU-9on various vegetables,including green peppers,sweet peppers,lettuce,potatoes,carrots,gourd and cabbages,were examined.The results showed that HBEU-9was strongly pathogenic toward a wide varieties of vegetables.Based on the morphological characteristics and the 16 S rDNA sequence analysis,the pathogen was identified to be Erwinia chrysanthemi（Ech）.The antagonism of 7antibiotics on the pathogen identified gentamicin as the most effective agent among all,even at a relatively low concentration of 60μg·mL^-1.

关 键 词：红椒 软腐病 分离鉴定 菊欧文氏菌 致病性 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]