偏肿栓菌内生木霉菌株18-21抑菌效果研究  

Biocontrol Effect of Trichoderma viridescens Strain 18-21 Screened from Fruiting Bodies of Trametes gibbosa

作　　者：李强[1] 李小林[2] 黄羽佳 陈诚[1] 黄文丽[1] 熊川[1] 郑林用[1] 

机构地区：[1]四川省农业科学院生物技术核技术研究所,四川成都610066 [2]四川省农业科学院土壤肥料研究所,四川成都610066 [3]四川省技术创新促进会,四川成都610017

出　　处：《微生物学杂志》2016年第1期17-21,共5页Journal of Microbiology

基　　金：四川省科技支撑计划项目(2013NZ0029;2014FZ0004)

摘　　要：为了筛选获得对植物根腐病及食用菌腐败等有效的生防菌,采用抑菌圈法和对峙培养法筛选生防菌株,通过形态学和16S r DNA分子鉴定确定生防菌分类地位。从1株野生偏肿栓菌Trametes gibbosa子实体中分离获得1株具有抑菌效果的生防菌,代号为18-21。筛选获得的这株生防菌对植物根腐病及食用菌腐败病原菌腐皮镰刀菌(Fusarium solani)、三线镰刀菌(Fusarium tricinctum)的抑制率分别为56.7%、65.8%,并对铜绿假单胞菌(Pseudomonas aeruginosa)、枯草芽胞杆菌(Bacillus subtilis)、侧芽胞杆菌(Bacillus latersprorus)、表皮葡萄球菌(Staphylococcus epidermidis)等病原细菌均具有显著的抑菌效果,通过形态学和分子生物学分类鉴定,确定为渐绿木霉(Trichoderma viridescens)。研究结果拓宽了生防菌的分离材料来源,丰富了生防资源,具有很大的应用潜力。In order to screen and obtain effective biocontrol microbes（ BM） against plant root rot and rot of edible fungi,inhibition ring and confrontation culture methods were used to screen the BM. The classification site of the BM were identified according to the morphology characters and ITS sequence analysis. One BM isolated from wild fruiting bodies of Trametes gibbosa and with the ability of inhibition the pathogen was obtained,coded name 18-21. The screened obtained BM had strong antagonism against plant root rot and edible fungi rot（ Fusarium solani and Fusarium tricinctum） with inhibition rate at 56. 7% and 65. 8% respectively. It also had significant inhibition effects against pathogens like Pseudomonas aeruginosa,Bacillus subtilis,Bacillus latersprorus,Staphylococcus epidermidis etc. The strain was identified and classified as Trichoderma viridescens through morphology,molecular biology. The results of the study had widened the isolation material source of BM and enriched biocontrol resources,showing promising potential applications.

关 键 词：偏肿栓菌 内生菌 生物防治 抑菌 镰刀菌 

分 类 号：Q939.92[生物学—微生物学]