雪莲果叶黄酮的纯化工艺  

Purification of Total Flavonoids from Yacon Leave

作　　者：陈红惠[1] 刘芳[1] 沈清清[2] 

机构地区：[1]云南文山学院化工学院,云南文山663000 [2]云南文山学院环境与资源学院,云南文山663000

出　　处：《食品研究与开发》2015年第21期45-49,共5页Food Research and Development

基　　金：云南省教育厅科学研究基金项目(2013Y581)

摘　　要：采用大孔树脂吸附法对雪莲果叶黄酮纯化工艺进行研究,选择9种大孔吸附树脂,通过其对雪莲果叶黄酮的静态吸附率和解吸率比较研究,筛选出HPD-100型树脂为较优的雪莲果叶黄酮的吸附树脂。最佳纯化工艺条件为:上样液质量浓度1.806 mg/mL,上样液p H2.0,上柱流速1 mL/min,以60%乙醇溶液为洗脱液,洗脱流速1 mL/min,洗脱体积为114 mL,解吸率为96.56%,该纯化工艺可使雪莲果叶粗提物中黄酮纯度由18.50%提高到56.24%。Macroporous adsorbing resin was used in purification flavonoids from yacon leaves, 9 macroporous resins were compared for difference through static adsorption and desorption experiments. HPD-100 resin was selected as a suitable material to purify the flavonoids from yacon leaves. The optimum conditions for separating and purifying flavonoids from yacon leaves were obtained. Results showed that the concentration of the sample1.806 mg/m L, p H 2.0,flow rate of sample solution 1 m L/min, with 60 % ethanol as eluting solvent, while the eluting speed 1 m L/min.,elution volume was 114 m L and desorption rate was 96.56 %. Under such conditions,the purity of flavonoids of yacon leaves from 18.50 % to 56.24 %.

关 键 词：雪莲果叶 黄酮 大孔树脂 纯化 

分 类 号：TQ464[化学工程—制药化工]