5-aza-cdr对低氧预适应神经细胞NG108的DNMTs表达的影响  

The effects of 5-aza-2'-deoxycytidine on the expression of DNMTs in hypoxic preconditioning NG108-15 cells

作　　者：孙明英[1,2] 张柱霞 杨洁[1,2] 姜树原[1] 王光丽[3] 张颜波[4] 邵国[1,2] 

机构地区：[1]包头医学院中心实验室生物医学研究中心,内蒙古包头014010 [2]包头医学院基础医学院,内蒙古包头014010 [3]丽水学院医学院,浙江丽水323000 [4]泰山医学院附属医院神经内科,山东泰安271000

出　　处：《泰山医学院学报》2015年第12期1321-1323,共3页Journal of Taishan Medical College

基　　金：国家自然科学基金(81060212;81160244;81360316;81460283);内蒙古自然科学基金资助项目(2010BS1104;2014MS0810);中国博士后基金项目(20080430851);内蒙古自治区高等学校青年科技英才支持计划资助(NJYT-13-A10);研究生科研创新项目[S201410127(Y02)];教育部留学回国人员科研启动基金(46批)

摘　　要：目的观察低氧预适应和DNA甲基转移酶(DNA methyltransferase,DNMT)抑制剂5-氮-2'脱氧胞苷(5-aza-2'deoxycytidine,5-aza-cdR)对神经细胞系NG108-15的DNMTs表达的影响,研究DNA甲基转移酶抑制剂对重复低氧NG08-15细胞的神经保护作用的可能分子机制。方法 NG08-15细胞随机分为药物处理组(5-aza-cdR)和正常组(control),每组又分为C组(未低氧)、H组(1%低氧处理8 h)和HP组(1%低氧/21%复氧4个循环后1%低氧预处理后低氧8h)处理,5-aza-cdR的浓度为10.0μM,Q-PCR检测DNMTs mRNA的表达。结果C,H和HP的DNMT1在5-aza-cdR和control组内和组间无显著性变化;在control组中,HP较H的DNMT3A mRNA表达降低(P<0.05),HP较C的DNMT3B mRNA降低(P<0.05);5-aza-cdR组中,H较C和HP的DNMT3B mRNA降低(P<0.05);HP的DNMT3B mRNA在5-aza-cdR组和control组之间有差异(P<0.05)。结论单纯低氧预适应可以使DNMT3A和DNMT3B mRNA表达降低,5-aza-cdR+低氧预适应可以影响DNMT3BmRNA的表达。Objective： The aim of this study was to observe the effects of 5- aza- 2＇– deoxycytidine on the DNMTs expression in nerval cell line NG108- 15 which repeatedly exposed to autoprogressive hypoxia and to investigate the possible neuroprotection mechanism in it. Methods： NG108- 15 cells were divided into 5- aza- cdR（ 10. 0 μmol / L） group and control group. Each group were treated with hypoxic（ H）,hypoxic preconditioning（ HP） and no hypoxia（ C）. The mRNA levels of DNMTs were analyzed by QPCR. Results： DNMT1 was not found difference between 5- aza- cdR treatment group and control group. Hypoxia preconditioning treatment can change the expression of DNMT3 A and DNMT3 B in control group. The DNMT3 A mRNA of HP group（ P〈0. 05 vs H） and the DNMT3 B mRNA of HP（ P〈0. 05 vs C） in control group. DNMT3 B mRNA of H was found to be decreased in 5- aza- cdR（ P〈0. 05 vs H and C）; DNMT3 B mRNA of HP was different between the 5- aza- cdR group and control group（ P〈0. 05）. Conclusion： Hypoxic preconditioning can change the expression of DNMT3 A and DNMT3 B mRNA. Hypoxic preconditioning combined with 5- aza- cdR can affect the expreesion of DNMT3 B mRNA.

关 键 词：低氧预适应 NG108-15细胞 5-氮-2'脱氧胞苷 DNA甲基转移酶 

分 类 号：R741[医药卫生—神经病学与精神病学]