纤维素酶辅助醇提荔枝核黄酮的工艺研究  

Study on Process of Cellulase Assisted Ethanol Extraction of Litchi Seed Flavonoids

作　　者：姚莉[1] 戴远威[1] 何敏[1] 吴丽[2] 韩淑琴[3] 

机构地区：[1]广东科贸职业学院生物技术系,广东广州510000 [2]华南农业大学食品学院,广东广州510000 [3]中山职业技术学院,广东中山528400

出　　处：《食品研究与开发》2015年第24期69-72,共4页Food Research and Development

摘　　要：以荔枝核为原料,研究纤维素酶辅助醇提荔枝核黄酮的工艺。考察酶添加量、酶解时间、醇提温度、醇提时间4个单因素对荔枝核黄酮提取率的影响,在单因素试验的基础上,选择四因素三水平进行正交试验优化工艺条件。结果表明,纤维素酶辅助醇提荔枝核黄酮的最优工艺条件为酶添加量5 000 U/g、酶解时间90 min、醇提温度70℃、醇提时间2.5 h,在此条件下,荔枝黄酮提取率可达5.64%,比乙醇回流法提取提高了74.07%。Used litchi seed as material, the process of cellulase assisted ethanol extraction of litchi seed flavonoids was on studied. The effects of enzymatic content, enzymolysis time, extraction temperature and extraction time on extraction rates of litchi seed flavonoids were explored, and four factors and three levels of orthogonal test was experimented based on single-factor test. The result showed that the optimal conditions of process of cellulase assisted ethanol extraction of Litchi seed flavonoids were enzymatic content of 5 000 U/g,enzymolysis time of 90 min, extraction temperature of 70 ℃ and extraction time of 2.5 h. Under these conditions, the extraction rates of litchi seed flavonoids could be up to 5.64 %, which was 74.07 % higher than that by ethanol extraction.

关 键 词：荔枝核黄酮 纤维素酶 醇提 

分 类 号：TQ28[化学工程—有机化工]