阿卡斑病毒云南分离株全基因组序列特征研究  

Characterization and Analyses of the Full-length Genome of a Strain of the Akabane Virus Isolated from Mosquitoes in Yunnan Province,China

作　　者：冯云[1] 章域震[1] 杨卫红[1] 张海林[1] 

机构地区：[1]云南省地方病防治所云南省自然疫源性疾病防控技术重点实验室,云南大理671000

出　　处：《病毒学报》2016年第2期161-169,共9页Chinese Journal of Virology

基　　金：NSFC-云南省联合基金/重点项目(No.U1036601)

摘　　要：为阐明分离自云南省的蚊媒阿卡斑病毒(Akabane virus,AKV)DHL10M110株(简称云南株)的分子生物学特征,采用RT-PCR和核苷酸序列测定方法,对云南株进行全基因组序列测定和分析。结果获得了云南株全基因组核苷酸序列,该病毒株的L、M和S基因核苷酸序列全长分别为6 869bp、4 309bp和856bp,其中开放读码框(Open reading frame,ORF)核苷酸序列依次为6 756bp、4 206bp和702bp,并分别编码2252、1402和234个氨基酸。这三个基因片段ORF的系统进化分析表明,云南株L基因与AKV标准株OBE-1(日本)和AKVS-7/SKR/2010株(韩国)处于同一进化分支;在M和S基因进化树中,云南株与日本、韩国和台湾地区的AKV流行株亲缘关系较近,但云南株单独处于一个小的进化分支。同源性分析表明,云南株L、M和S片段ORF与OBE-1株的核苷酸(氨基酸)同源性分别为92.6%(98%)、88.5%(94%)和96.4%(99.1%)。云南株与OBE-1株的L、M和S片段分别有45、84和2个氨基酸位点的差异。此外,无论L、M和S基因核苷酸序列的同源性或系统进化分析均表明,云南株与肯尼亚和澳大利亚AKV分离株进化关系较远,同源性较低。本研究进一步通过病毒全基因组序列分析证实DHL10M110病毒株为AKV,属亚洲进化群,但与日本和韩国株相比仍有一定差异,云南株地域特征明显。这是首次在中国大陆地区测定到该病毒的全基因组序列。We wished to sequence the full-length genomes of the DHL10M110 strain of the Akabane virus（AKV）isolated from mosquitoes in Yunnan Province,China,in 2010.We also wished to analyze the characteristics of these complete nucleotide sequences.The complete genomic sequence of the DHL10M110 strain from Yunnan Province was obtained by reverse transcription-polymerase chain reaction and direct sequencing.We found that the length of the L,M and S gene nucleotide sequences of the DHL10M110 strain were 6 869-bp,4 309-bp and 856-bp,respectively,including the open reading frame（ORF）nucleotide sequences of 6 756-bp（L）,4 206-bp（M）and 702-bp（S）,encoding 2252,1402 and 234amino-acid polyproteins,respectively.Phylogenetic analyses based on L-fragment ORF showed that the DHL10M110 strain had a close relationship with the OBE-1strain of the AKV from Japan and AKVS-7/SKR/2010 strain of the AKV from South Korea.Phylogenetic analyses based on M-and S-fragment ORF showed that the DHL10M110 strain had a close relationship with the epidemic strains of the AKV from Japan,South Korea and Taiwan,but that the DHL10M110 strain had a lone evolutionary branch.In terms of nucleotide（amino acid）homology,the similarity of L-,M-and S-fragment ORFs of the DHL10M110 strain to the OBE-1strain from Japan was 92.6%（98%）,88.5%（94%）and 96.4%（99.1%）,respectively.When comparing the DHL10M110 strain with the OBE-1strain,we noted 45,84,and 2different sites in the amino acids of L,M and S fragments,respectively.Homology and phylogenetic analyses also suggested that the DHL10M110 strain had a distant relationship with the epidemic strains of the AKV from Kenya and Australia.Also,we confirmed by complete genomic sequence analyses that the DHL10M110 strain was cladeAsia of the AKV.However,differences between the DHL10M110 strain compared with strains from Japan and South Korea were also noted.These results suggest that the DHL10M110 strain harbored relatively stable genetic characteristics and distinct regional features.This is th

关 键 词：阿卡斑病毒 全基因组 系统进化分析 同源性分析 

分 类 号：R373.3[医药卫生—病原生物学]