赤金蛋白T30替代南美白对虾饲料中鱼粉的效果研究  

Study on effects of replacement of fish meal with pure-gold peptide T30 of white shrimp

作　　者：刘清兵[1,2,3] 曹启猛 于跃芹[1] 王雷[2,3] 蒋克勇[2,3] 刘梅[2,3] 王宝杰[2,3] 张维金 李燕翠 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042 [2]中国科学院实验海洋生物学重点实验室,山东青岛266071 [3]青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室,山东青岛266071 [4]秦皇岛益尔生物推广服务有限公司,河北秦皇岛066000

出　　处：《饲料工业》2016年第6期26-31,共6页Feed Industry

基　　金：国家科技支撑计划课题[2012BAD17B03]

摘　　要：研究旨在探讨赤金蛋白T30替代饲料中不同水平的鱼粉对南美白对虾生长指标、营养成分、消化酶活力和血清免疫指标的影响。赤金蛋白T30分别替代饲料中鱼粉用量的15%(B组)和30%(C组),对照(A组)为商品化南美白对虾饲料。饲养周期60 d。结果表明:喂养20 d,B组的末体重、增重率、特定生长率显著高于A、C组(P<0.05);喂养40 d,B组的末体重和增重率显著高于A、C组(P<0.05);喂养60 d,B组的末体重、增重率、特定生长率最高。虾体的粗蛋白含量C组>B组>A组(P<0.05);粗脂肪含量A组>B组>C组(P<0.05);各组灰分和水分含量差异不显著(P>0.05)。蛋白酶、纤维素酶和脂肪酶活力差异不显著(P>0.05);C、B组淀粉酶活力显著高于A组(P<0.05)。B组AKP活力显著高于A、C组(P<0.05);B组SOD活力显著高于A组(P<0.05);各组ACP和LZM活力差异不显著(P>0.05)。试验结果表明:赤金蛋白T30替代饲料中一定用量的鱼粉可以显著促进南美白对虾的生长,改善虾体营养成分,显著提高部分消化酶活力和血清免疫指标,且替代水平15%的生长效果优于替代水平30%。综合考虑其效果与经济性,建议用赤金蛋白T30替代南美白对虾饲料里鱼粉用量的15%。This study was conducted to investigate the effects of different level pure-gold peptide T30 substitution for fish meal in the feed on growth indices, nutritive composition, digestive enzyme, and se-rum immune indices of white shrimp. The consumption of fish meal in the feed were replaced by pure-gold peptide T30, 15%（group B）, 30%（group C） respectively, and commercial feed of white shrimp asthe control（group A）. The feeding cycle was 60 days. The results showed that： after feeding for 20 days, the final body weight（FBW）, the weight gain ratio（WGR）, the specific growth ratio（SGR） of groupB were significantly higher than group A and group C（P〈0.05）; after feeding for 40 days, the FBW andWGR of group B were significantly higher than group A and group C（P〈0.05）; after feeding for 60 days, the FBW, WGR and SGR of group B were the highest among the three groups. The crude pro-tein content of the white shrimp showed significant difference among the three groups（P〈0.05）, andgroup C〉B〉A; The crude lipid of group A was higher than group B and group B was higher than C（A〉B〉C） with significant difference（P〈0.05）; ash and moisture content showed no significant difference（P〉0.05）. The activities of protease, cellulose and lipase showed no significant difference among the groups（P〈0.05）; the activity of amylase of group C andB was significantly higher than group A（P〈0.05）.The activity of alkaline phosphatase（AKP） ofgroup B was significantly higher than othergroups（P〈0.05）; the activity of total superoxidedismutase（SOD） of group B was higher than group C（P〈0.05）; the activity of acid phosphatase（ACP） and lysozyme showed no significant differenceamong the three groups（P〉0.05）. Result showed that： the replacement of fish meal with pure-gold pep-tide T30 in a certain amount could significantly promote the growth and improve the nutrients of thewhite shrimp, and the activities of part of the digestive enzymes and serum im

关 键 词：南美白对虾 赤金蛋白T30 生长指标 营养成分 消化酶活力 血清免疫指标 

分 类 号：S963.73[农业科学—水产养殖]