mmunomodulatory properties of silver nanoparticles ontribute to anticancer strategy for murine fibrosarcoma  

作　　者：Biswajit Chakraborty Ramkrishna Pal Mohammed Ali Leichombam Mohindro Singh Dewan Shahidur Rahman Sujit Kumar Ghosh Mahuya Sengupta 

机构地区：[1]Immunobiology Laboratory, Department of Biotechnology, Assam University, Silchar, India [2]Department of Chemistry, Assam University, Silchar, India

出　　处：《Cellular & Molecular Immunology》2016年第2期191-205,共15页中国免疫学杂志（英文版）

摘　　要：The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles （AgNPs） have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin （MSA）-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses （1-10 mg/kg b.w.） were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg （IC50： 6.15 mg/kg b.w.） sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9- and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-α, IL-6 and IL-1β were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA.The use of nanotechnology in nanoparticle-based cancer therapeutics is gaining impetus due to the unique biophysical properties of nanoparticles at the quantum level. Silver nanoparticles （AgNPs） have been reported as one type of potent therapeutic nanoparticles. The present study is aimed to determine the effect of AgNPs in arresting the growth of a murine fibrosarcoma by a reductive mechanism. Initially, a bioavailability study showed that mouse serum albumin （MSA）-coated AgNPs have enhanced uptake; therefore, toxicity studies of AgNP-MSA at 10 different doses （1-10 mg/kg b.w.） were performed in LACA mice by measuring the complete blood count, lipid profile and histological parameters. The complete blood count, lipid profile and histological parameter results showed that the doses from 2 to 8 mg （IC50： 6.15 mg/kg b.w.） sequentially increased the count of leukocytes, lymphocytes and granulocytes, whereas the 9- and 10-mg doses showed conclusive toxicity. In an antitumor study, the incidence and size of fibrosarcoma were reduced or delayed when murine fibrosarcoma groups were treated by AgNP-MSA. Transmission electron micrographs showed that considerable uptake of AgNP-MSA by the sentinel immune cells associated with tumor tissue and a morphologically buckled structure of the immune cells containing AgNP-MSA. Because the toxicity studies revealed a relationship between AgNPs and immune function, the protumorigenic cytokines TNF-α, IL-6 and IL-1β were also assayed in AgNP-MSA-treated and non-treated fibrosarcoma groups, and these cytokines were found to be downregulated after treatment with AgNP-MSA.

关 键 词：AgNP-MSA FIBROSARCOMA IL-1β IL-6 IMMUNOMODULATION nanoparticles TNF-α 

分 类 号：S858.237.4[农业科学—临床兽医学] TB383[农业科学—兽医学]