同种异体来源骨髓间充质干细胞移植可改善衰老心脏的功能  

Allogeneic bone marrow mesenchymal stem cell transplantation can improve the function of the aging heart

作　　者：李艳菊[1] 丁元廷[2] 周媛[2] 王飞清 曾强武[2] 安仕刚[2] 刘洋[2] 

机构地区：[1]贵州医科大学附属医院,贵州省贵阳市550004 [2]贵阳中医学院第一附属医院,贵州省贵阳市550002

出　　处：《中国组织工程研究》2016年第6期814-819,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:目前认为骨髓间充质干细胞能在损伤局部反应性分泌各种各样的因子,具有促进细胞增殖,抑制细胞凋亡的作用。目的:观察骨髓间充质干细胞对衰老心脏的治疗作用并探讨其可能的作用机制。方法:30只SD大鼠随机分为3组:正常空白组,阳性造模组,治疗组,后2组给予D-半乳糖皮下注射制作大鼠衰老模型,治疗组经大鼠尾静脉注射同种异体骨髓间充质干细胞,每周1次,共4次;末次注射后1周制作心脏组织切片,苏木精-伊红染色观察各组大鼠心脏组织学改变;免疫印记法检测各组心脏组织中碱性成纤维细胞生长因子的表达,Real-time PCR检测心脏组织中p53 m RNA的表达。结果与结论:治疗组与阳性造模组相比,能够改善衰老心脏的病理形态;与阳性造模组相比,治疗组能够使心脏组织碱性成纤维细胞生长因子表达升高(P<0.05),p53 m RNA表达减低(P<0.05),推测骨髓间充质干细胞可能和心脏细胞相互作用分泌碱性成纤维细胞生长因子、降低p53基因的表达从而具有治疗衰老心脏的作用。BACKGROUND： Bone marrow mesenchymal stem cells can secrete a variety of factors in the local lesion, and these factors can promote cell proliferation and inhibit cell apoptosis. OBJECTIVE： To observe the curative effect of bone marrow mesenchymal stem cell transplantation on the aging heart of rats and to explore the possible mechanism of action. METHODS： Thirty Sprague-Dawley rats were randomized into three groups： normal blank group, model group and treatment group. Aging models were made in the latter two groups by injection of D-galactose. Rats in the treatment group were given allogeneic bone marrow mesenchymal stem cell injection, once a week, totally four times. At 1 week after final injection, the heart tissues were sliced into sections to observe the pathological changes using hematoxylin-eosin staining. Western blot assay was used to detect the expression of basic fibroblast growth factor in the heart tissues. Real-time PCR was used to measure the expression of p53 m RNA in the heart tissues. RESULTS AND CONCLUSION： Bone marrow mesenchymal stem cell transplantation could improve the pathological morphology of the aging heart. Compared with the model group, the expression of basic fibroblast growth factor in the heart tissues was significantly higher in the treatment group（P〈0.05）, but the m RNA expression of p53 was lower（P〈0.05）. It is speculated that bone marrow mesenchymal stem cells can interact with heart cells to secrete basic fibroblast growth factor and reduce p53 m RNA expression, thereby playing a curative effect on the aging heart.

关 键 词：干细胞 移植 骨髓间充质干细胞 衰老 心脏 碱性成纤维细胞生长因子 P53 

分 类 号：R394.2[医药卫生—医学遗传学]