骨髓间充质干细胞移植联合氯化锂治疗兔股骨头缺血坏死疗效  

Bone marrow mesenchymal stem cell transplantation combined with lithium chloride treatment for avascular necrosis of the femoral head in rabbits

作　　者：李巍[1] 户小伟[2] 冼呈 劳山[1] 

机构地区：[1]广西医科大学第一附属医院骨关节外科,广西壮族自治区南宁市530021 [2]河南省安阳市第三人民医院外二一科,河南省安阳市455000

出　　处：《中国组织工程研究》2016年第6期868-875,共8页Chinese Journal of Tissue Engineering Research

基　　金：广西医疗卫生重点科研课题(重2010014)~~

摘　　要：背景:氯化锂促进骨髓间充质干细胞增殖并促进股骨头缺血坏死区成骨能力的研究日益受到关注。目的:比较移植骨髓间充质干细胞联合氯化锂修复股骨头坏死外的优势。方法:1取出生1周的新西兰白兔第2代的骨髓间充质干细胞,以加入含0,5,10,20,40 mmol/L氯化锂培养。2选取48只健成年新西兰大白兔,用液氮冷冻方法制成右侧股骨头坏死模型,随机分为4组,单纯造模组:不植入任何材料;氯化锂组:造模3 d后每日应用氯化锂;骨髓间充质干细胞移植组:造模后用明胶海绵植入股骨头内,制成骨髓间充质干细胞悬液注入股骨头内;骨髓间充质干细胞移植+氯化锂组:在骨髓间充质干细胞移植组的基础上每日应用氯化锂;氯化锂的用法为每天45.2 mg/kg腹腔注射,术后3 d开始,连续应用4周。结果与结论:1以10 mmol/L的浓度对进兔骨髓间充质干细胞的增殖起最大作用,超过10 mmol/L的氯化锂促进作用开始下降。2股骨头坏死模型兔经干细胞联合氯化锂治疗后,形态有所恢复,骨密度增加,骨小梁增粗,股骨头中β-连环蛋白表达水平增加,且效果优于单纯干细胞或氯化锂治疗。3说明骨髓间充质干细胞移植联合氯化锂可以促进股骨头坏死区骨小梁的增生骨量增加骨密度增高,促进股骨头坏死的修复。BACKGROUND： Lithium chloride can promote the proliferation and osteogenic capacity of bone marrow mesenchymal stem cells in the necrotic region after avascular necrosis of the femoral head, which has become an issue of concern. OBJECTIVE： To compare the advantages and disadvantages of bone marrow stem cell transplantation combined with lithium chloride in the treatment of rabbit femoral head necrosis. METHODS： Passage 2 bone marrow mesenchymal stem cells from 1-week-old New Zealand rabbits were cultured in 0, 5, 10, 20, 40 mmol/L lithium chloride. Forty-eight healthy adult New Zealand rabbits were selected to make femoral head necrosis models in the right femoral head using liquid nitrogen freezing method and then randomized into four groups： model group with no implantation; lithium chloride group given lithium chloride treatment at 3 days after modeling; cell transplantation group given gelatin sponge implantation and bone marrow mesenchymal stem cell suspension injection into the femoral head after modeling; combined group given bone marrow mesenchymal stem cell suspension injection and lithium chloride treatment. Intraperitoneal injection of lithium chloride（45.2 mg/kg） was given daily beginning at the postoperative 3rd day, and the treatment duration was 4 weeks. RESULTS AND CONCLUSION： Lithium chloride at 10 mmol/L had the maximum effect on the proliferation of rabbit bone marrow mesenchymal stem cells, and if the concentration of lithium chloride was 10 mmol/L, the promotion role of lithium chloride began to decline. After combined treatment, the morphology of the femoral head was restored a little, with increased bone density and thickened trabecular bone; the level of β-catenin in the femoral head was significantly increased in the combined group compared with the cell transplantation group or the lithium chloride group. These findings show that bone marrow stem cell transplantation combined with lithium chloride treatment can promote the recovery from femoral head necrosis by increasing b

关 键 词：干细胞 移植 氯化锂 骨髓间充质干细胞 Β-连环蛋白 股骨头坏死 兔 WNT信号通路 骨小梁 

分 类 号：R394.2[医药卫生—医学遗传学]