早期运动训练联合神经干细胞移植对脊髓损伤模型大鼠后肢运动功能的影响  

Early exercise training combined with neural stem cell transplantation improves hindlimb motor function after spinal cord injury in rats

作　　者：武玉江[1] 侯英诺[1] 张子檀[2] 刘中坡[1] 聂志红[1] 樊格林 

机构地区：[1]邢台市人民医院脊柱骨科,河北省邢台市054001 [2]邢台市人民医院麻醉科,河北省邢台市054001

出　　处：《中国组织工程研究》2016年第6期876-882,共7页Chinese Journal of Tissue Engineering Research

基　　金：河北省科技计划项目(12277772)~~

摘　　要：背景:研究表明,神经干细胞移植联合运动训练可以促进脊髓损伤大鼠后肢运动功能的恢复,但其作用机制尚未完全阐明。目的:观察早期运动训练联合神经干细胞移植对脊髓损伤大鼠后肢运动功能恢复的影响。方法:60只SD大鼠建立脊髓损伤模型后随机分为3组:对照组20只,损伤后即刻给予常规处理;细胞移植组20只,损伤后给予神经干细胞移植;实验组20只,损伤后给予神经干细胞移植的同时联合早期运动训练。分别于损伤前及损伤后的1,7,14,21 d,通过BBB评分及斜板实验评估大鼠后肢运动功能的恢复情况;损伤后第14天利用Western blot法检测损伤脊髓组织caspase-3和髓过氧化物酶的表达;损伤后第21天做常规苏木精-伊红染色切片,观察大鼠脊髓损伤组织结构改变。结果与结论:1各组大鼠在损伤后第7,14,21天的BBB评分及斜板实验评分,细胞移植组优于对照组,实验组优于细胞移植组,各组间比较差异有显著性意义(P<0.05)。2损伤后14 d对照组大鼠caspase-3表达明显升高,髓过氧化物酶表达也明显升高,细胞移植组大鼠caspase-3和髓过氧化物酶的表达均高于实验组(P<0.05)。3细胞移植组脊髓病理炎性反应减轻,实验组炎性反应进一步减轻,损伤区域结构改善,脊髓组织结构相对清晰、趋于完整。4神经干细胞移植联合早期运动训练能有效改善脊髓运动功能,其可能通过降低caspase-3和髓过氧化物酶的表达,减轻对脊髓组织的继发性损害,以利于维持脊髓运动神经元功能。BACKGROUND： Studies have shown that neural stem cell transplantation combined with exercise training can promote the recovery of hindlimb motor function from spinal cord injury in rats, but its mechanism of action has not been fully elucidated. OBJECTIVE： To investigate the effects of early exercise training combined with neural stem cell transplantation on the recovery of hindlimb motor function in rats with spinal cord injury. METHODS： Sixty Sprague-Dawley rats with spinal cord injury were randomly divided into three groups： control group（n=20, given conventional treatment after injury）, cell transplantation group（n=20, given neural stem cell transplantation after injury）, experimental group,（n=20, given neural stem cell transplantation combined with early exercise training after injury）. Recovery of the hindlimb motor function was assessed by Basso, Beattie and Bresnahan scale and inclined plane test before and at 1, 7, 14, 21 days after injury. Western blot assay was used to detect caspase-3 and myeloperoxidase expression. Hematoxylin-eosin staining was done at 21 days after injury to observe the structure changes of the injured spinal cord. RESULTS AND CONCLUSION：（1） Scores of Basso, Beattie and Bresnahan scale and inclined plane test were significantly better in the experimental group than the cell transplantation group followed by the control group（P〈0.05）.（2） In the control group, the expression of caspase-3 and myeloperoxidase was significantly increased at 14 days after injury. In the cell transplantation, the expression of caspase-3 and myeloperoxidase was significantly higher than the experimental group（P〈0.05）.（3） Pathological inflammation was reduced most in the experimental group followed by the cell transplantation group. In the experimental group, the structure of injured spinal cord was improved and became relatively clear and intact. These findings indicate that neural stem cell transplantation combined with early exercise training can effectively p

关 键 词：干细胞 移植 神经干细胞 脊髓损伤 大鼠 运动功能 

分 类 号：R394.2[医药卫生—医学遗传学]