内皮祖细胞移植联合早期运动改善脊髓损伤区血管再生及后肢功能  

Endothelial progenitor cell transplantation combined with early exercise training for spinal cord injury: improvement in hindlimb function and angiogenesis in the injured region

作　　者：赵素香[1] 侯英诺[1] 张子檀[2] 刘中坡[1] 聂志红[1] 樊格林 

机构地区：[1]邢台市人民医院脊柱骨科,河北省邢台市054001 [2]邢台市人民医院麻醉科,河北省邢台市054001

出　　处：《中国组织工程研究》2016年第6期883-890,共8页Chinese Journal of Tissue Engineering Research

摘　　要：背景：内皮祖细胞广泛应用于各种血管疾病治疗中,早期运动训练有助于脊髓损伤后运动功能的恢复,但单独的内皮祖细胞移植或早期运动训练然治疗效果目前还不甚理想。目的：观察内皮祖细胞移植联合早期运动训练对大鼠脊髓损伤区血管再生及后肢功能的影响。方法：将80只成年SD大鼠按改良Allen打击法建立脊髓损伤模型后,随机分为4组。①模型组大鼠尾静脉注入培养液。②内皮祖细胞组大鼠经尾静脉泵注内皮祖细胞（3×10^6个）。③运动组大鼠采用滚筒训练和跑台训练两种运动功能训练方法训练2周。④联合组大鼠尾静脉注射内皮祖细胞后,采用滚筒训练和跑台训练2周。结果与结论：①移植后2周：联合组大鼠下肢运动功能和脊髓病理损伤恢复程度优于内皮祖细胞组及运动组,联合组大鼠CM-Dil阳性细胞比例、辣根过氧化物酶阳性神经纤维数量和毛细血管密度以及血管内皮生长因子和脑源性神经营养因子表达水平高于内皮祖细胞组及运动组。②提示早期运动训练对脊髓损伤起到神经保护的作用,内皮祖细胞移植的同时联合早期运动训练能够促进脊髓损伤大鼠神经突触和血管的再生,改善其大鼠肢体运动功能,其机制可能与升高损伤区血管内皮生长因子和脑源性神经营养因子表达有关。BACKGROUND： Endothelial progenitor cells are widely used in the treatment of various vascular diseases, and early exercise training contributes to restore motor function after spinal cord injury. However, the therapeutic effects of endothelial progenitor cell transplantation or early exercise training alone are unfavorable. OBJECTIVE： To observe the influence of transplantation of endothelial progenitor cells combined with early exercise training on blood vessel regeneration and hind limb function in rats after spinal cord injury. METHODS： Eighty adult Sprague-Dawley rats were enrolled to establish spinal cord injury models using the modified Allen＇s method, and then randomly divided into four groups. Rats were respectively given culture medium via the tail vein, injection of endothelial progenitor cells（3×10^6） via the tail vein, roller and treadmill trainings for 2 weeks, or injection of endothelial progenitor cells via the tail vein followed by 2 weeks of roller and treadmill trainings in the model, cell transplantation, exercise and combined groups. RESULTS AND CONCLUSION： At 2 weeks after transplantation, the hindlimb motor function of rats in the combined group was better than that in the cell transplantation group and exercise group, and moreover, the percentage of CM-Dil positive cells, the number of horseradish peroxidase-positive nerve fibers, capillary density and expression of vascular endothelial growth factor and brain-derived neurotrophic factor were also significantly higher in the combined group than the cell transplantation group and exercise group. These findings indicate that early exercise training has a neuroprotective role in spinal cord injury; endothelial progenitor cell transplantation combined with early exercise training can promote regeneration of synapses and blood vessels and improve hindlimb motor function of rats, probably by increasing expression levels of vascular endothelial growth factor and brain-derived neurotrophic factor.

关 键 词：干细胞 移植 内皮祖细胞 早期运动训练 脊髓损伤 血管再生 功能 神经修复 

分 类 号：R394.2[医药卫生—医学遗传学]