维吾尔药铁力木不同极性部位体外抗氧化作用的研究  

Antioxidant activities of different polarity chemical constituents from Mesua ferrea in vitro

作　　者：马俊鹏[1] 王新玲[2] 李芸[3] 胡君萍[2] 王小青[2] 丁燕楠 王晓梅[2] 

机构地区：[1]新疆维吾尔自治区人民医院米东医院药械科,新疆乌鲁木齐830000 [2]新疆医科大学药学院,新疆乌鲁木齐830011 [3]新疆生产建设兵团第五师医院药剂科,新疆博乐833400

出　　处：《安徽医药》2016年第3期421-424,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家"十二五"科技支撑计划(No 2012BAI30B02)

摘　　要：目的 研究维吾尔药铁力木不同极性部位的体外抗氧化活性。方法 以Vc为对照,通过测定铁力木乙醇提取物、水提取物及乙醇提取物石油醚（沸程60-90℃）、乙酸乙酯、正丁醇、水各萃取物对Fe^3＋的还原能力、对DPPH自由基（DPPH·）和超氧自由基（O2^-）的清除能力,研究铁力木的体外抗氧化活性。结果 铁力木各部位对Fe^3＋均表现出一定的还原能力,对DPPH·及O2^-均表现出一定的清除作用,但作用均弱于VC。其中水层及正丁醇层对Fe^3＋的还原能力较强,而其石油醚层和乙酸乙酯层还原能力较弱;水层和水提取物对DPPH·具有一定的清除能力;石油醚层及水提取物清除O2^-的作用较强。结论铁力木具有较好的抗氧化能力。Objective To study the antioxidant activity of the different polarity chemical constituents from the buds of Mesua ferrea. Methods The capacity of ferric reducing antioxidant power and scavenging DPPH·,O2^- of the ethanol total extract,water extract,pe-troleum ether fraction,ethylacetate fraction,n -butanol fraction and water fraction from the buds of Mesua ferrea were evaluated by Fe^3＋ reduction capacity experiments and DPPH·,O2^- scavenging assay in vitro at different concentrations.The results were compared with those of positive control vitamin C （VC ）.Results The ferric reducing antioxidant power and DPPH·,O2^- scavenging activity of dif-ferent polarity chemical constituents from the buds of Mesua ferrea were weaker than those of VC .Among them,the water fraction and n-butanol fraction exhibited strong reduction power of Fe^3＋.The water extract and water fraction demonstrated strong scavenging activi-ties of DPPH·.The petroleum ether fraction and water extract demonstrated the strong scavenging activities of O2^- .Conclusions Mesua ferrea has a strong antioxidant activity.

关 键 词：铁力木 抗氧化活性 铁离子还原反应 DPPH自由基 超氧自由基 

分 类 号：R29[医药卫生—民族医学]