肝细胞癌多基因甲基化研究  

Multi gene promotion in hepatocellular carcinoma

作　　者：程越 张长松[2] 郭献灵[2] 卫立辛[2] 

机构地区：[1]河源市人民医院,广东河源517000 [2]第二军医大学东方肝胆外科医院,上海200433

出　　处：《分子影像学杂志》2016年第1期33-36,共4页Journal of Molecular Imaging

摘　　要：目的通过研究肝癌中多基因启动子区的甲基化,说明抑癌基因甲基化在肝癌发生的普遍性。方法分别抽提冻存的肝癌、癌旁组织和正常肝组织的DNA,利用亚硫酸氢钠具有使未甲基化的胞嘧啶转为尿嘧啶而不改变甲基化胞嘧啶的特性分别设计甲基化引物和非甲基化引物,然后分别进行PCR反应。我们分别检测了转移抑制基因p15,SYK,TIMP-3,E-cadherin,RASSAF1和肿瘤相关基因p53,RB1,WT1,p14,p16启动子区在60个肝癌、癌旁和6个正常肝组织的甲基化情况。结果 60个肝癌标本中10个基因的甲基化情况各不相同：从p53的8%~90%的RASSAF1;肝癌组织比癌旁组织的甲基化平均水平高,二者在统计学上有显著差异（P〈0.05）。结论肝癌中有多个基因存在甲基化,且肝癌组织甲基化水平较癌旁组织高。Objective To explain the aberrant methylation of tumor suppressor genes is common hepatocellular carcinoma.Methods Extracting cryopreservation of hepatocellular carcinoma,paracancerous tissues and normal liver tissues of DNA,using sodium bisulfite has the characteristics of unmethylated cytosine to uracil without changing the methylated cytosine methylation primers were designed and the non methylated primers,and then respectively by PCR reaction.We examined the metastasis suppressor gene p15,SYK,TIMP-3,E-cadherin,RASSAF1 and tumor related genes p53,RB1,WT1,P14,p16 promoter methylation status of the promoter region in 60 liver cancer,para cancer and 6 normal liver tissues.Results The frequency of promoter methylation of ten genes in 60 HCC varied from 8% in P53 to 90% in p14.The average methylated-gene numbers were significantly different between HCC and paired non-tumor tissues（P〈0.05）.Conclusion There are multiple gene methylation in HCC exist,and the level of methylation of liver cancer tissue than in noncancerous tissue high.

关 键 词：肝细胞癌 基因 甲基化 

分 类 号：R735.7[医药卫生—肿瘤]