烟草BY-2细胞生长和呼吸对不同类型酸雨的响应  

T he response of tobacco BY- 2 cells on weight and respiration under sim ulated acid rain of different types of treatm ents

作　　者：刘鹏[1] 逄好胜 张萌萌[1] 张秀丽[1] 孙广玉[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《黑龙江科学》2016年第8期10-13,共4页Heilongjiang Science

基　　金：黑龙江省自然科学基金重点项目(ZD2011-05)

摘　　要：为了直观探究不同类型酸雨对植物生理生长的影响,以烟草Bright Yellow-2(BY-2)细胞为试验材料,模拟不同p H值(1.5、2.5、3.5、4.5、5.5)下硫酸型酸雨(SAR)、硝酸型酸雨(NAR)及混合型酸雨(MAR),以期了解其对烟草BY-2细胞干重和呼吸的影响。研究表明,在不同酸度下,烟草细胞干重均低于对照,细胞呼吸出现紊乱变化,说明不同p H下的酸雨对细胞均有伤害,BY-2细胞的干重和呼吸有不良反馈,且不同酸度的伤害程度不一,强酸(p H值为1.5)下对细胞迫害性最强。因此,不同类型酸雨对植物有迫害性,迫害性大小为SAR>NAR>MAR。To understand the effects of different types of acid rain on plant growth and physiological, Tabacco bright yellow-2（BY-2） cells were used as the experimental materials to study the effects simulated sulfuric acid rain （SAR）, nitric acid rain（NAR） and the mixed acid rain（MAR） on physiological characteristics in different pH values（1.5,2.5,3.5,4.5,5.5）, the results showed that the dry weight of cells decline to compare with control in different acidities, The cellular respiration appeared unstable situation, the content of DNA is different in DNA images, illustrated that simulated acid rain is harmful to cells, and had bad influence of BY-2 cells on weight and respiration, strong acid （pH 1.5） is the most harmful and persecution is different in different acidities. Conclusion： acid rain of different types are harmful to plants, the persecution： SAR〉NAR〉MAR.

关 键 词：酸雨 烟草BY-2细胞 PH 细胞干重 细胞呼吸 

分 类 号：Q814[生物学—生物工程]