经宿主致倦库蚊幼虫复壮后灭蚊真菌贵阳腐霉的毒力观察  

An Observation on Virulence of Mosquito-killing Fungus,Pythium guiyangense after Rejuvenated through Its Host,Culex quinquefaciatus Larvae

作　　者：杨虓 苏晓庆[1,2] 

机构地区：[1]珠海盈嘉行科技有限公司,广东珠海519000 [2]贵州医科大学生物学教研室,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第3期261-264,共4页Journal of Guiyang Medical College

基　　金：国家自然科学基金资助项目(No:30760140)

摘　　要：目的:经宿主致倦库蚊幼虫复壮后灭蚊真菌贵阳腐霉(Pg)的毒力观察。方法:向高压灭菌碗加入致倦库蚊幼虫和毒力减退的Pg菌丝,记录蚊虫死亡数和感染数,计算死亡率和感染率;取被感染的蚊虫用水琼脂平板、KPYG2平板和SFE平板分离Pg,用新分离的Pg感染致倦库蚊幼虫,记录复壮后最高组感染率、单碗的最高和最低蚊虫感染率,观察Pg感染蚊虫毒力的变化。结果:复壮后最高组Pg对蚊虫平均感染率、单碗Pg对蚊虫最高感染率及单碗Pg对蚊虫最低感染率均达到40%或以上,单碗Pg最高感染率甚至达100%。结论:采用宿主致倦库蚊幼虫复壮毒力减退的Pg菌丝,可提高Pg灭蚊的毒力。Objective： To observe the virulence of mosquito-killing fungus,Pythium guiyangense（ Pg） after being rejuvenated through its host,Culex quinquefaciatus larvae. Methods： Bioassay was conducted by adding Pg mycelia with its host C. quinquefaciatus larvae into disinfected plastic containers,recording numbers of dead and infected larvae,and calculating mortality rates and infection rates.Pg was re-isolated from infected larvae using KPYG2,SFE and water agar plates,and used to infect C. quinquefaciatus larvae again. The mortality rates and infection rates,specially infection rates in groups and in individual containers were calculated and compared. Results： After several times of continuous rejuvenation,C. quinquefaciatus larvae infection rates by Pg increased markedly,including the highest group infection rates and the highest and lowest infection rates in individual containers,which were generally up to 40% or above,specially,an infection rate of 100% was achieved in individual container. Conclusion： Rejuvenation through host is an effective way to increase virulence of Pg to mosquitoes.

关 键 词：致倦库蚊 幼虫 复壮 生物灭蚊 贵阳腐霉 毒力 

分 类 号：R184.31[医药卫生—流行病学] R379[医药卫生—公共卫生与预防医学]