不同来源茶树组织的茶多酚含量比较  

Comparative Study on Content of Tea Polyphenols Extracted from Different Tea Plant Tissue

作　　者：吕倩[1] 涂云华 李剑[1] 刘涛华[1] 张荣庆[1] 罗垚[1] 康颖倩[1] 

机构地区：[1]贵州医科大学微生物学教研室 [2]贵州医科大学附院皮肤性病科,贵州贵阳550004

出　　处：《贵阳医学院学报》2016年第3期265-267,共3页Journal of Guiyang Medical College

基　　金：国家自然基金地方项目(31060006;31260029);贵州省社会发展科技攻关项目[黔科合SY字(2011)3017号];贵州省卫生厅科技项目(gzwkj2010-1-025);贵阳市科技局社会发展与民生计划[筑科合同(2011103)16号]

摘　　要：目的:比较不同茶树组织中茶多酚的含量,为茶树修剪枝叶的利用提供参考。方法:通过生石灰水沉淀法提取绿茶树的新鲜叶、干燥叶、新鲜树枝、干燥树枝的茶多酚粗提取物和纯化茶多酚,比较4种茶树组织的茶多酚粗提取物质量及提取率;采用分光光度计法测定标准茶多酚吸光度(A)值,绘制茶多酚标准曲线,通过4种茶树组织纯化茶多酚的吸光度(A)值在标准曲线上获得茶多酚含量并计算其纯度,比较4种茶树组织纯化茶多酚含量和纯度。结果:茶树组织茶多酚粗提取物质量及提取率从高到低依次为新鲜叶、干燥叶、新鲜树枝及干燥树枝,茶多酚含量从高到低依次为新鲜叶、干燥叶、新鲜树枝及干燥树枝,纯度从高到低依次则为新鲜叶、新鲜树枝、干燥叶及干燥树枝。结论:新鲜茶叶和新鲜茶树枝的茶多酚含量较高,可为茶叶及茶树枝综合开发利用提供参考。Objective： To compare the tea polyphenols amount extracted from different tea plant tissure,and to provide the scientific reference for full development and utilization of tea branches and tea leaves. Methods： The tea polyphenols from four different tea plant tissue（ fresh tea leaves,dry tea leaves,fresh tea branch and dry tea branch） were extracted by limewater precipitation method. The quality of tea polyphenols crude extract and rate of extraction were compared between 4 different tea plant tissue. Spectrophotometry was adopted to determine absorbance of tea polyphenols of standard concentration and the standard curve of tea polyphenols was plotted,by which the tea polyphenols content and purity of 4 different tea plant tissue was calculated and compared. Results： In 4 different tea plant tissue,the quality of tea polyphenols crude extract and rate of extraction from high to low in order were fresh tea leaves,dry tea leaves,fresh tea branch and dry tea branch,the tea polyphenols content from high to low in order were fresh tea leaves,dry tea leaves,fresh tea branch and dry tea branch,and the tea polyphenols purity from high to low in order were fresh tea leaves,fresh tea branch,dry tea leaves and dry tea branch. Conclusion： The tea polyphenols content in fresh tea leaves and fresh tea branch is relatively high. The study provides the scientific reference for full development and utilization of tea branches and tea leaves.

关 键 词：茶 茶多酚 生石灰水沉淀法 萃取 茶树组织 分光光度法 

分 类 号：Q657.8[生物学—生物物理学]