膈俞穴注射4T1细胞构建小鼠乳腺癌模型的实验研究  

Experimental Study on Construction of Mice Breast Cancer Model By Geshu Acupoint Injection with 4T1 cells

作　　者：赵大尉 谢甦[2] 张意 管志敏[1] 贺昀[1] 张雲杰 

机构地区：[1]贵州医科大学研究生院 [2]贵州医科大学附院中医科,贵州贵阳550004 [3]天津市静海区陈官屯医院中医科,天津静海301600

出　　处：《贵阳医学院学报》2016年第3期304-306,309,共4页Journal of Guiyang Medical College

基　　金：贵州省中医药管理局中医药;民族医药科学技术研究课题基金(QZYY-2015-155)

摘　　要：目的：探讨膈俞穴注射乳腺癌4T1细胞构建小鼠乳腺癌模型的可行性。方法：清洁级4~6周龄雌性BALB/c小鼠16只,随机分为膈俞穴组和右肩胛组各8只,膈俞穴组将小鼠乳腺癌4T1细胞悬液接种于BALB/c小鼠左侧膈俞穴,右肩胛组接种于右侧肩胛部作为对照,观察两组小鼠成模时间、成瘤率、小鼠的远期存活率以及肿瘤生长曲线。结果：膈俞穴组小鼠的成瘤时间快于右肩胛组,差异有统计学意义（P〈0.05）;肿瘤生长曲线显示膈俞穴组肿瘤生长速度明显高于右肩胛组,且后期两组之间的差距越来越明显（P〈0.05）,但两组成瘤率及远期存活率均无差异。结论：膈俞穴注射乳腺癌4T1细胞可成功复制小鼠乳腺癌模型,且成模时间短。Objective： To explore the feasibility of mouse breast cancer model construction by Geshu acupoint injection of breast cancer 4T1 cells. Methods： Sixty female SPF BALB / c mice of 4 to 6weeks old were randomly divided into Geshu group and Shoulder blade group,8 mice in each group.Geshu group was inoculated with breast cancer 4T1 cells suspension in left Geshu acupoint while Shoulder blade group inoculated with breast cancer 4T1 cells suspension in right shoulder blade.Tumor formation time,tumor formation rate,tumor growth curve and forward survival rate were compared between two groups. Results： Tumor formation time in Geshu group was significantly shorter than that of Shoulder blade group（ P〈0. 05）. The tumor growth curve showed that tumor growth rate in Geshu group was significantly higher that that of Shoulder blade group,and the difference in tumor growth rate between the two groups was becoming more and more obvious（ P〈0. 05）. Howerver,there was no difference in the tumor formation rate and the forward survival rate. Conclusion： Ge Shu acupoint injection of breast cancer 4T1 cells can successfully construct mouse breast cancer model in relatively short time.

关 键 词：膈俞穴 乳腺肿瘤 4T1细胞 模型 动物 小鼠 

分 类 号：R73-351[医药卫生—肿瘤]