模拟微重力对恶性胶质瘤细胞U87影响的实验研究  

Experimental Study of Effect of Modeled Microgravity on Malignant Glioblastoma Cell U87

作　　者：石子璇 李军昌[1] 李哲[1] 谢娟[1] 刘倩 杜志超[4] 曹亮[1] 张莎[1] 王宗仁[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032 [2]陕西省中医医院,陕西西安710003 [3]巨野县中医医院儿科,山东菏泽274900 [4]第四军医大学生化教研室,陕西西安7100324

出　　处：《现代生物医学进展》2016年第8期1411-1414,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81273879)

摘　　要：目的:探讨模拟微重力(Modeled microgravity,MMG)对恶性胶质瘤细胞U87形态、生长增殖、基质金属蛋白酶-2、-9(Matrix metalloproteinase 2,MMP-2、Matrix metalloproteinase 9,MMP-9)及神经胶质酸性蛋白(Glial fibrillary acidic protein,GFAP)表达的影响。方法:常规培养U87细胞,传代培养3代后分为两组,正常重力组(Normal gravity,NG组)及模拟微重力干预组(Modeled microgravity,MMG组),相应条件下培养24 h,48 h和72 h。倒置显微镜观察U87细胞形态改变,细胞计数法及四甲基偶氮唑盐微量酶反应比色法(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT法)检测模拟微重力干预和干预后U87细胞生长增殖情况;Western Blot检测不同培养时间后胶质瘤细胞U87 MMP-2、MMP-9及GFAP蛋白的表达情况。结果:模拟微重力条件下培养72 h后,U87细胞轮廓不规则,边缘圆钝,触角变少;模拟微重力条件下,U87细胞生长速度明显减慢,并且经模拟微重力干预48 h和72 h后的胶质瘤细胞经传代再培养,其增殖率也明显低于正常重力组;同时,U87细胞MMP-2及MMP-9蛋白表达水平与模拟微重力处理时间呈时间依赖性下降,而GFAP蛋白表达水平则呈时间依赖性升高。结论:模拟微重力影响U87细胞的形态、生长及表型。To investigate the effect of modeled microgravity on morphology, proliferation and the protein expression of Matrix metalloproteinase 2（MMP-2）, Matrix metalloproteinase 9（MMP-9）and Glial fibrillary acidic protein（GFAP）of a glioma cell line U87. Methods： After cultured for 3 passages, U87 cells were divided into two groups： normal gravity group（NG） and modeled microgravity group（MMG）, and cultured for 24 h, 48 h and 72 h in corresponding conditions. The changes of morphology were observed by inverted microscope; the cytometry assay and 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）assay were used to evaluate the growth rate of the U87 cells under or after MMG treatment; Western Blot was applied to analyze the MMP-2, MMP-9 and GFAP protein expression of the U87 cells. Results： After cultured for 72 h under MMG condition, the morphology of U87 cells changed into being irregular and turned to be acuity; under MMG condition, the growth rate of the U87 cells was significantly slowed down,especially for 48 h and 72 h, compared with that of U87 cells under NG condition. Furthermore, after cultured under MMG condition for48 h and 72 h not for 24 h, the sub-cultured U87 cells grew more slowly under NG condition, compared those of NG group; Additionally,the expression of MMP-2 and MMP-9 of U87 cells was reduced in time-dependent after exposed to MMG. Nevertheless, the expression of GFAP was up-regulated under MMG condition. Conclusion： MMG could change the morphology, proliferation and phenotype of U87 cells.

关 键 词：模拟微重力 胶质瘤细胞 增殖 基质金属蛋白酶 恶性表型 

分 类 号：R739.41[医药卫生—肿瘤]