熊去氧胆酸对肝硬化大鼠血清IL-6、MMP-3、TIMP-1及胃肠Cajal细胞c-kit表达的影响  

Effect of Ursodesoxycholic Acid on the IL-6, MMP-3 and TIMP-1 Levels in Serum and c-kit Expression in Cajal Cells of Rats with Liver Cirrhosis

作　　者：王燕燕[1] 许小红[2] 李彦 赵敏 张忠群[5] 

机构地区：[1]贵阳护理职业学院,贵州贵阳550023 [2]贵阳市第一人民医院,贵州贵阳550002 [3]贵州省第二人民医院,贵州贵阳550004 [4]解放军第四十四医院,贵州贵阳550000 [5]贵阳市肺科医院,贵州贵阳550006

出　　处：《现代生物医学进展》2016年第8期1435-1438,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30360098)

摘　　要：目的:探讨熊去氧胆酸对肝硬化大鼠血清IL-6、MMP-3、TIMP-1水平及胃肠Cajal细胞c-kit表达的影响。方法:选取72只大鼠,随机分为四组,每组18只。空白组给予正常饲养,其余三组均注射CCl_4予酒精和高蛋白饮食,熊去氧胆酸组大鼠注射熊去氧胆酸1.5 mg/Kg·d;吗丁啉组给予吗丁啉0.85 mg/Kg·d;模型组仅给予注射生理盐水。治疗结束后,对比各组血清IL-6、MMP-3、TIMP-1水平及胃肠Cajal细胞c-kit含量。结果:与空白组比较,模型组大鼠血清IL-6、MMP-3、TIMP-1水平均明显升高(P<0.01),胃肠Cajal细胞c-kit含量显著降低(P<0.01)。与模型组相比,熊去氧胆酸组与吗丁啉组大鼠血清IL-6、MMP-3、TIMP-1水平均显著降低(P<0.01),小肠推进率增高(P<0.01),胃肠Cajal细胞c-kit含量显著升高(P<0.01);与吗丁啉组相比,熊去氧胆酸组血清IL-6、MMP-3、TIMP-1水平较低(P<0.01),小肠推进率、胃肠Cajal细胞c-kit含量显著升高(P<0.01)。结论:熊去氧胆酸对肝硬化具有较好疗效,可改善肝纤维化和胃肠功能,这可能与其降低血清IL-6、MMP-3、TIMP-1水平及提高胃肠Cajal细胞C-kit含量有关。To investigate the effect of ursodesoxycholic acid on the IL-6, MMP-3, and TIMP-1 in serum and c-kit expression in Cajal cells of rats with liver cirrhosis. Methods： 72 rats were selected and randomly divided into four groups. The blank group were get normal feeding and others group were treated with CCl_4 alcohol and high-protein diet. The ursodesoxycholic acid group was treated with 1.5 mg/Kg·d ursodesoxycholic acid. The domperidone group was treated with 0.85 mg/Kg·d domperidone. The model group was treated with physiological saline. Then the serum levels of MMP-3, TIMP-1 and IL-6 and expression of c-kit in gastrointestinal Cajal cells of different groups were observed and compared. Results： Compared with the blank group, the serum levels of IL-6, MMP-3,TIMP-1 were higher（P〈0.01）, the C-kit expression in gastrointestinal Cajal cells was lower（P〈0.01）. Compared with the model group,the serum IL-6, MMP-3, TIMP-1 levels were lower in the ursodesoxycholic acid group and the domperidone group（P〈0.01）, the intestinal propulsion rate and the C-kit expression in gastrointestinal Cajal cells were higher（P〈0.01）. Conclusion： Ursodesoxycholic acid had good curative effect in the treatment of liver cirrhosis, which could improve the liver fibrosis and gastrointestinal function.

关 键 词：肝硬化 IL-6 MMP-3 TIMP-1 胃肠Cajal细胞 

分 类 号：R575.2[医药卫生—消化系统] R-33[医药卫生—内科学]