武汉地区非小细胞肺癌EGFR基因突变相关性研究  

Correlation research of EGFR gene mutation in non-small cell lung cancer in Wuhan area

作　　者：周泉[1] 李艳[1] 童永清[1] 

机构地区：[1]武汉大学人民医院检验科,湖北武汉430060

出　　处：《职业与健康》2016年第5期617-623,共7页Occupation and Health

基　　金：国家临床重点专科建设项目(财社[2010]305号)

摘　　要：目的调查武汉地区非小细胞肺癌(NSCLC)患者表皮生长因子受体(EGFR)基因型分布特点,探究肺癌组织EGFR基因突变与患者吸烟习惯、肿瘤病理学类型、肿瘤分化程度以及淋巴结转移与否的相关性。方法收集185例NSCLC患者石蜡组织切片,采用常规聚合酶链反应(PCR)方法检测EGFR基因18、19、20、21四个外显子,DNA测序分析确定EGFR基因突变类型。结果 185例NSCLC患者中,男性EGFR突变率为7.3%,明显低于女性的17.8%,差异有统计学意义(P<0.05);吸烟患者FGFR基因突变率为7.7%,明显低于不吸烟者的20.0%,差异有统计学意义(P<0.01),其中腺癌EGFR突变率为15.3%(18/118),腺鳞癌突变率为10.0%(3/30),鳞癌突变率为5.4%(2/37);共检测出7种类型突变,分别为Gln787Gln、Leu858Arg、E746-A750del、S752-I759del、A750-I759del、Gly719Ser、Ser768Ile。结论武汉地区NSCLC EGFR基因的突变形式主要为点突变及缺失突变。EGFR基因突变与患者性别有关,与肿瘤病理学类型、肿瘤分化程度和淋巴结转移与否无明显关系。[Objective] To survey the distribution characteristics of genotypes of epidermal growth factor receptor （EGFR） in patients with non-small cell lung cancer （NSCLC） in Wuhan area, explore the correlation between the living habits, tumor pathology type, tumor differentiation degree, lymphatic metastasis and EGFR mutation in NSCLC patients.[Methods]The paraffin tissue sections were collected from 185 NSCLC patients, to detect four exons of 18, 19, 20 and 21 in EGFR gene by using the conventional PCR method. DNA sequencing analysis was used to determine the types of mutations in EGFR gene.[Results] Among 185 NSCLC patients, the EGFR mutation rate of male patients（7.3% ） was significantly lower than thatof female patients, and the difference was statistically significant （P〈0.05）. The EGFR mutation rate of smoking patients （7.7%） was significantly lower than that in never-smoking patients, and the difference was statistically significant （P〈0.01）. The EGFR mutation rate of adenocarcinoma, adenosquamous and squamous carcinoma was 15.3% （18/118）, 10.0% （3/30） and 5.4% （2/37）, respectively. There were 7 types of mutations in EGFR gene, which was Gln 787Gln, Leu858Arg, E746-A750del, S752-I759 del, A750-I759 del, Gly719 Set and Ser 768Ile, respectively. [Conclusion] The main mutation types of EGFR gene in NSCLC patients are point mutation and deletion mutation in Wuhan area. EGFR gene mutation is associated with the sex of patients, and it has no correlation with tumor pathology type, tumor differentiation degree the lymphatic metastasis.

关 键 词：表皮生长因子受体(ECFR) 基因突变 非小细胞肺癌中国图书资料 

分 类 号：R734.2[医药卫生—肿瘤]