非小细胞肺癌组织中p-mTOR、p70s6k的表达变化及其临床意义  

Expression of p-m TOR and p70s6k in non-small-cell lung cancer and its clinical significance

作　　者：许浩然[1] 吕艳超[2] 韩双双[3] 王丽红[3] 岳洪娟[3] 王嘉[3] 

机构地区：[1]承德市中心医院,河北承德067000 [2]保定市第一中心医院总院 [3]河北医科大学第一医院

出　　处：《山东医药》2016年第11期11-13,共3页Shandong Medical Journal

基　　金：河北省医学科学研究重点课题计划项目(20090333);河北省科技计划自筹经费项目(152777233)

摘　　要：目的观察非小细胞肺癌组织中磷酸化雷帕霉素靶蛋白(p-m TOR)及核糖体40s小亚基S6蛋白激酶(p70s6k)蛋白的表达变化,并探讨其意义。方法非小细胞肺癌患者82例,手术切除肿瘤组织作为肺癌组,取患者肺癌同侧远端的肺泡正常组织46例份作为对照组1,正常支气管黏膜组织50例份为对照组2。免疫组化方法检测3组组织中的p-m TOR及p70s6k蛋白,并分析其与非小细胞肺癌临床病理参数的关系。结果肺癌组、对照组1、对照组2 p-m TOR蛋白表达阳性率分别为68.29%、17.39%、32.0%,p70s6k蛋白表达阳性率分别为85.37%、21.74%、32.00%,肺癌组与对照组1、对照组2相比,P均<0.01。非小细胞肺癌低分化p-m TOR蛋白表达阳性率高于中高分化(P<0.01)。肺癌组织中p-m TOR和p70s6k蛋白的表达呈正相关(r=0.21,P=0.003 2)。结论p-m TOR、p70s6k蛋白在非小细胞肺癌组织中高表达,可作为早期诊断肺癌的指标或抗癌药物作用的新靶点。Objective To observe the expression changes of phosphorylated mammalian target of rapamycin（ pm TOR） and p70s6 k protein in non-small-cell lung cancer（ NSCLC） tissues and to discuss its clinical significance. Methods Eighty-two cases of patients with NSCLC were selected. The tumor tissues obtained from surgery（ lung cancer group）,46 cases of tissue samples from alveolus healthy tissues（ control group 1）,and 50 cases of tissue samples from bronchus mucosal tissues（ control group 2） were collected. The expression of p-m TOR and p70s6 k protein in the three groups was measured by immunohistochemistry and we analyzed the relationships with the clinical and pathological data.Results The positive expression rates of p-m TOR protein in the lung cancer group,control group 1 and control group 2were 68. 29%,17. 39% and 32. 0%,respectively. The positive expression rates of p70s6 k protein in the lung cancer group,control group 1 and control group 2 were 85. 37%,21. 74% and 32. 00%,respectively. Significant difference was found between the lung cancer group and the control group 1,control group 2（ all P〈0. 01）. The expression of p-m TOR protein in poorly differentiated cancer tissues was significantly higher than that of medium and high differentiation cancer tissues（ P〈0. 01）. In the NSCLC tissues,the p-m TOR and p70s6 k expression was positively correlated（ r = 0. 210,P =0. 003 2）. Conclusion p-m TOR and p70s6 k protein is highly expressed in NSCLC tissues,which may be used as the early-warning index in the early diagnosis or as a new target for anti-cancer drug.

关 键 词：肺肿瘤 非小细胞肺癌 磷酸化雷帕霉素靶蛋白 核糖体40s小亚基S6蛋白激酶 

分 类 号：R734.2[医药卫生—肿瘤]