细胞蜡块宫颈液基细胞P16在检测宫颈肿瘤中的作用  

The dectection of P16 in cervical tumor saved in wax block by cervical fluid cytology

作　　者：刘镜文 陈莉[2] 吴秋良[3] 

机构地区：[1]广东省惠州市第一妇幼保健院病理科,广东惠州516000 [2]广东省东莞市虎门医院妇产科,广东东莞523900 [3]广东省广州市中山大学肿瘤防治中心病理科,广东广州510060

出　　处：《分子诊断与治疗杂志》2016年第2期114-118,共5页Journal of Molecular Diagnostics and Therapy

摘　　要：目的探讨细胞蜡块P16检测在宫颈液基细胞学中的应用价值。方法采用标准化病人(standardized patient,SP)免疫组织化学的方法对180例细胞学标本进行P16检测。结果 P16在未见上皮内病变细胞(negative for intraepithelial lesion or malignancy,NILM)、未明确诊断意义的非典型鳞状上皮细胞(atypical squamous cell of undetermined significance,ASC-US)、不除外高度病变的非典型鳞状上皮细胞(atypical squamous cells,cannot exclude a high-grade squamous intraepithelial lesion,ASC-H)、低度鳞状上皮内病变(low-grade squamous intraepithelial lesion,LSIL)、高度鳞状上皮内病变(high-grade squamous intraepithelial lesion,HSIL)和鳞状细胞癌(squamous cell carcinoma,SCC)中的阳性率分别为0%、5.63%、60.00%、30.23%、100.00%和100.00%。在180例患者中,只有73例有组织活检的结果,其中P16阳性的为48例,宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)CINII级及以上病变患者为33例;P16阴性的有25例,无CINII级及以上病变。结论利用细胞蜡块P16免疫组织化学染色,显示P16的表达与宫颈癌的发生和发展相关,因此可用于宫颈癌的病变分级,也可作为辅助诊断指标应用于宫颈病变的筛查。Objective To evaluate the significance of the expression of P16 in cellular wax block liquid based cytology of cervical screening. Methods Standardized patient（SP） immunohistochemistry detection of protein P16 was performed on 180 specimens. Results The positive rates of P16 in negative for intraepithelial lesion or malignancy（NILM）, atypical squamous cell of undetermined significance（ASCUS）, atypical squamous cells, cannot exclude a high-grade squamous intraepithelial lesion（ASC-H）, low-grade squamous intraepithelial lesion（LSIL）, high- grade squamous intraepithelial lesion（HSIL） and squamous cell carcinoma（SCC） were 0%、5.63%、60.00%、30.23%、100.00% and 100.00%, respectively. There were only 73 patients who received the results of the biopsy, including 48 positive cases of P16 [with 33 cases of cervical intraepithelial neoplasia Ⅱ（CIN Ⅱ） or above] and 15 negative cases of P16（with no CIN Ⅱ or above）.Conclusion Cellular wax block P16 immunohistochemistry staining revealed that the expression of P16 protein was connected with the occurrence and development of cervical cancer, which may be used in the lesion classification of cervical cancer, and used as the auxiliary diagnostic indexes in the screening of cervical lesions.

关 键 词：宫颈肿瘤 液基细胞学 免疫组织化学 细胞蜡块 P16 

分 类 号：R737.33[医药卫生—肿瘤]