大鼠场景性恐惧记忆再激活过程中海马HDAC2的表达变化  

The expression of hippocampal HDAC2 during contextual fear memory reactivation in rats

作　　者：张娟[1] 黄伏连[1] 钟小林[1] 胡招兰 王洪涛[1] 张文娟[1] 乔小青[1] 黄艳青[1] 李芳[1] 李昌琪[1] 

机构地区：[1]中南大学基础医学院人体解剖与神经生物学系,长沙410013

出　　处：《神经解剖学杂志》2016年第2期154-160,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(31371212;81471372)

摘　　要：目的:探讨组蛋白去乙酰化酶2(histone deacetylase 2,HDAC2)在场景性恐惧记忆再激活过程中的表达变化。方法:对大鼠进行不可逃避的电击刺激与中性刺激(训练环境)联结匹配训练,建立大鼠场景性恐惧条件化模型,之后采用免疫组织化学、Western Blot和RT-PCR方法检测实验组(恐惧记忆唤起后30 min、2 h、6 h、24 h组和不进行恐惧记忆唤起的no-recall组)和对照组(与实验组相同的处理方式但没有足底电击的刺激)大鼠海马中HDAC2的表达变化。结果:免疫组织化学、Western Blot和RT-PCR方法检测结果均显示场景性恐惧记忆唤起后30 min与24 h,HDAC2在大鼠海马中的表达水平明显高于对照组,差异具有统计学意义。其它时间点及no-recall组HDAC2的表达与对照组相比,没有统计学意义。结论:场景性恐惧记忆唤起后30 min与24 h,HDAC2在大鼠海马中表达水平显著升高,此变化可能与恐惧记忆产生的相关行为(恐惧记忆的消退行为、焦虑行为等)有一定的联系。Objective： The present study is to explore the expression of hippocampal histone deacetylase2（HDAC2）during contextual fear memory reactivation in rats. Methods： The contextual fear memory in rats was established by using inescapable electric shocks pairing with a neutral stimulus（ training context）. The expression of HDAC2 in the hippocampus was investigated in the control and the exprimental group（30 min,2 h,6 h,24 h after fear memory recall,and norecall group） by immunohistochemistry,Western Blot and PT-PCR. Results： The expression of HDAC2 in the hippocampus was significantly increased compared with the control group at 30 min and 24 h after fear memory recall. It showed no significant differences between the control,no-recall group and other time points groups. Conclusion： The expression of HDAC2 in the hippocampus are significantly higher than that of control group 30 min and 24 h after fear memory recall which may be associated with fear memory relevant behavior（ e. g,anxiety,the extinction of fear memory）.

关 键 词：PTSD HDAC2 场景性恐惧记忆再激活 大鼠 

分 类 号：R749.5[医药卫生—神经病学与精神病学]